beta-Catenin (Cadherin associated protein, Beta 1 88kD, Catenin beta-1, CATNB, CHBCAT, CTNNB1)

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

beta-Catenin (Cadherin associated protein, Beta 1 88kD, Catenin beta-1, CATNB, CHBCAT, CTNNB1)

catalog :

138503

quantity :

20 ug

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

138503

Product wo Prefix :

beta-Catenin (Cadherin associated protein, Beta 1 88kD, Catenin beta-1, CATNB, CHBCAT, CTNNB1)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

20 ug

Isotype :

IgG

Desc1 :

Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, beta-catenin is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution of beta-catenin throughout the cytoplasm results in a diffuse cytoplasmic pattern. Immuno-staining of beta-catenin and E-cadherin is helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic beta-catenin staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas. Applications: Suitable for use in Western Blot, Flow Cytometry, Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 1-2ug/ml/30min @ RT Flow Cytometry: 0.5-1ug/10e6 cells Immunofluorescence: 1-2ug/ml Immunoprecipitation: 1-2ug/500ug protein lysate Optimal dilutions to be determined by the researcher. Positive Control: HeLa or MCF-7 cells. Breast carcinoma Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mo

Immunogen :

Synthetic peptide corresponding to the middle of human beta-Catenin (p120).

Specificity :

Recognizes human beta-catenin (p120). Species Crossreactivity: mouse.

Purity :

Purified by Protein A affinity chromatography.

Form :

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138503-AF.

Concentration :

~0.2mg/ml

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138503AF. Purity: Purified by Protein A affinity chromatography. Immunogen: Synthetic peptide corresponding to the middle of human beta-Catenin (p120). Specificity: Recognizes human beta-catenin (p120). Species Crossreactivity: mouse. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

FC IF IHC IP WB

Storage Temperature :

-20°C

Reference :

1. Dabbs DJ et. al. Am J Surg Path. 2007;31:427-437. 2. Sarrio D et. al. Oncogene. 2004;23:3272-3283. 3. Mastracci TL et. al. Mod Path. 2005;18:741-751.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/27/138503_1jpeg