This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
US Biological
product type :
antibody
product name :
Carcinoembryonic Antigen (Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, CEACAM5, CD66e)
catalog :
138502
quantity :
20 ug
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
COL-1

The same clone is also sold as:
reactivity :
human
product information
Catalog Number :
138502
Product wo Prefix :
Carcinoembryonic Antigen (Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, CEACAM5, CD66e)
Host :
mouse
Product Type :
Mab
Category :
Antibodies
Size1 :
20 ug
Clone # USB :
COL-1
Isotype :
IgG2a,k
Desc1 :
CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This monoclonal antibody does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix. Applications: Suitable for use in ELISA, Immunoprecipitation, Immunofluorescence, Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT Flow Cytometry: 0.5-1ug/10e6 cells Immunofluorescence: 1-2ug/ml Immunoprecipitation: 1-2ug/500ug protein lysate Optimal dilutions to be determined by the researcher. Positive Control: MCF7 or 293T cells. Colon carcinoma Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Calc Crossreactivity :
Hu
Immunogen :
Human colon carcinoma extract
Specificity :
Recognizes human COL-1. This antibody recognizes proteins of 80-200kD, identified as different members of CEA family.
Purity :
Purified by Protein A/G affinity chromatography.
Form :
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138502-AF.
Concentration :
~0.2mg/ml
Desc2 :
Product Type: Mab Isotype: IgG2a,k Clone No: COL-1 Host: mouse Source: human Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138502-AF. Purity: Purified by Protein A/G affinity chromatography. Immunogen: Human colon carcinoma extract Specificity: Recognizes human COL-1. This antibody recognizes proteins of 80-200kD, identified as different members of CEA family. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
E FC IF IHC IP WB
Storage Temperature :
-20°C
Reference :
1. Muraro R, et. al. Cancer Research, 1985, 45:5769-80. 2. Siler K, et. al. Biotechnology Therapeutics, 1993, 4(3-4):163-81. 3. Robbins PF, et. al. International Journal of Cancer, 1993, 53(6):892-7. 4. Shi ZR, et. al. Journal of Histochemistry and Cytochemistry, 1994, 42(9):1215-9.
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/21/138502_1.jpg
company information
US Biological
4 Technology Way
Salem, MA01970
service@usbio.net
https://www.usbio.net
800-520-3011
headquarters: USA
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