Bcl-X (Apoptosis regulator Bcl-X, B cell lymphoma 2 like, BCL XL/S, Bcl-2-like protein 1, Bcl2-L-1, BCL2L, Bcl2l1, BCLX, BclXL, BclXs, Protein phosphatase 1 regulatory subunit 52 (PPP1R52))

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Bcl-X (Apoptosis regulator Bcl-X, B cell lymphoma 2 like, BCL XL/S, Bcl-2-like protein 1, Bcl2-L-1, BCL2L, Bcl2l1, BCLX, BclXL, BclXs, Protein phosphatase 1 regulatory subunit 52 (PPP1R52))

catalog :

138499

quantity :

20 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

BX006

The same clone is also sold as:

OriGene: AM32834PU-T, AM32834PU-S
Abnova: MAB11266
Novus Biologicals: NBP2-33164, NBP2-25240

reactivity :

human

product information

Catalog Number :

138499

Product wo Prefix :

Bcl-X (Apoptosis regulator Bcl-X, B cell lymphoma 2 like, BCL XL/S, Bcl-2-like protein 1, Bcl2-L-1, BCL2L, Bcl2l1, BCLX, BclXL, BclXs, Protein phosphatase 1 regulatory subunit 52 (PPP1R52))

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

20 ug

Clone # USB :

BX006

Isotype :

IgG2a

Desc1 :

Recognizes a protein of 27kD, identified as the Bcl-X protein. This monoclonal antibody shows no cross-reaction with Bcl-2a or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241aa protein which suppresses cell death. And Bcl-XS (short), a 178aa protein lacking a 63aa domain which functions as a dominant inhibitor of Bcl-2. This MAb reacts with both Bcl-XS and Bcl-XL proteins. Applications: Suitable for use in Flow Cytometry, Immunofluorescence, ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/10e6 cells Western Blot: 0.5-1ug/ml Immunofluorescence: 1-2ug/ml Immunoprecipitation: 1-2ug/500ug protein lysate Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT Optimal dilutions to be determined by the researcher. Positive Control: Jurkat, K562, HL-60, or HeLa Cells. Reed-Sternberg cells in Hodgkin’s lymphoma. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mo Po Rt

Immunogen :

Synthetic peptide corresponding to aa3-14 (Cys-QSNRELVVDFLS) from human Bcl-X.

Specificity :

Recognizes human Bcl-X, ~27kD. Species Crossreactivity: mouse, rat and porcine.

Purity :

Purified by Protein A/G affinity chromatography.

Form :

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138499-AF.

Concentration :

~0.2mg/ml

Desc2 :

Product Type: Mab Isotype: IgG2a Clone No: BX006 Host: mouse Source: human Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138499AF. Purity: Purified by Protein A/G affinity chromatography. Immunogen: Synthetic peptide corresponding to aa3-14 (Cys-QSNRELVVDFLS) from human Bcl-X. Specificity: Recognizes human Bcl-X, ~27kD. Species Crossreactivity: mouse, rat and porcine. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E FC IF IHC IP WB

Storage Temperature :

-20°C

Reference :

1. Hsu YT, et. al. Journal of Biological Chemistry, 1997, 272(21):13829-34. 2. Hsu YT, et. al. PNAS, 1997, 94(8):3668-72. 3. Wolter KG, et. al. Journal of Cell Biology, 1997, 139(5):1281-92.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/4/138499_1.jpg