This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
US Biological
product type :
antibody
product name :
Bcl-2 (Apoptosis Regulator Bcl-2, B Cell CLL/lymphoma-2)
catalog :
138498
quantity :
20 ug
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
8C8

The same clone is also sold as:
reactivity :
human
product information
Catalog Number :
138498
Product wo Prefix :
Bcl-2 (Apoptosis Regulator Bcl-2, B Cell CLL/lymphoma-2)
Host :
mouse
Product Type :
Mab
Category :
Antibodies
Size1 :
20 ug
Clone # USB :
8C8
Isotype :
IgG1,k
Desc1 :
This antibody recognizes a protein of 25-26kD, identified as the bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative. Applications: Suitable for use in ELISA, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml Flow Cytometry: 0.5-1ug/10e6 cells Immunofluorescence: 1-2ug/ml Immunoprecipitation: 1-2ug/500ug protein lysate Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT Optimal dilutions to be determined by the researcher. Positive Control: Jurkat, K562, HL-60, or HeLa Cells. Tonsil or follicular lymphomas. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Calc Crossreactivity :
Hu Mk Po
Immunogen :
Synthetic peptide corresponding to aa41-54 (GAAPAPGIFSSQPG-Cys) from human Bcl-2.
Specificity :
Recognizes human Bcl-2. Species Crossreactivity: monkey and porcine. Does not react with mouse or rat.
Purity :
Purified by Protein A/G affinity chromatography.
Form :
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138498-AF.
Concentration :
~0.2mg/ml
Desc2 :
Product Type: Mab Isotype: IgG1,k Clone No: 8C8 Host: mouse Source: human Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138498AF. Purity: Purified by Protein A/G affinity chromatography. Immunogen: Synthetic peptide corresponding to aa41-54 (GAAPAPGIFSSQPG-Cys) from human Bcl-2. Specificity: Recognizes human Bcl-2. Species Crossreactivity: monkey and porcine. Does not react with mouse or rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
E FC IF IHC IP WB
Storage Temperature :
-20°C
Reference :
1. Wolter KG, et. al. Journal of Cell Biology, 1997, 139(5):1281-92.
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/48/138498_1jpeg
company information
US Biological
4 Technology Way
Salem, MA01970
service@usbio.net
https://www.usbio.net
800-520-3011
headquarters: USA
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