This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
US Biological
product type :
antibody
product name :
Bcl-2 (Apoptosis Regulator Bcl-2, B Cell CLL/lymphoma-2)
catalog :
138497
quantity :
20 ug
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
100/D5

The same clone is also sold as:
reactivity :
human
product information
Catalog Number :
138497
Product wo Prefix :
Bcl-2 (Apoptosis Regulator Bcl-2, B Cell CLL/lymphoma-2)
Host :
mouse
Product Type :
Mab
Category :
Antibodies
Size1 :
20 ug
Clone # USB :
100/D5
Isotype :
IgG1,k
Desc1 :
This antibody recognizes a protein of 25-26kD, identified as the bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative. Applications: Suitable for use in ELISA, Flow Cytometry, Western Blot, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/10e6 cells Western Blot: 0.5-1ug/ml Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT Immunofluorescence: 1-2ug/ml Immunoprecipitation: 1-2ug/500ug protein lysate Optimal dilutions to be determined by the researcher. Positive Control: Jurkat, K562, HL-60, or HeLa Cells. Tonsil or follicular lymphomas. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Calc Crossreactivity :
Hu
Immunogen :
Synthetic peptide corresponding to aa41-54 (GAAPAPGIFSSQPG-Cys) from human Bcl-2.
Specificity :
Recognizes human Bcl-2. Does not react with mouse or rat.
Purity :
Purified by Protein A/G affinity chromatography.
Form :
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138497-AF.
Concentration :
~0.2mg/ml
Desc2 :
Product Type: Mab Isotype: IgG1,k Clone No: 100/D5 Host: mouse Source: human Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138497AF. Purity: Purified by Protein A/G affinity chromatography. Immunogen: Synthetic peptide corresponding to aa41-54 (GAAPAPGIFSSQPG-Cys) from human Bcl-2. Specificity: Recognizes human Bcl-2. Does not react with mouse or rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
E FC IF IHC IP WB
Storage Temperature :
-20°C
Reference :
1. Pezzella F et. al. American Journal of Pathology,1990, 137(2):225-32.
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/42/138497_1jpeg
company information
US Biological
4 Technology Way
Salem, MA01970
service@usbio.net
https://www.usbio.net
800-520-3011
headquarters: USA
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