This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
US Biological
product type :
antibody
product name :
Bax (BCL2-associated X protein, Apoptosis regulator BAX, BAX protein, BCL2L4, Bcl-2-like Protein 4, Bcl2-L-4)
catalog :
138495
quantity :
20 ug
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
2D2

The same clone is also sold as:
reactivity :
human
product information
Catalog Number :
138495
Product wo Prefix :
Bax (BCL2-associated X protein, Apoptosis regulator BAX, BAX protein, BCL2L4, Bcl-2-like Protein 4, Bcl2-L-4)
Host :
mouse
Product Type :
Mab
Category :
Antibodies
Size1 :
20 ug
Clone # USB :
2D2
Isotype :
IgG1
Desc1 :
Recognizes a protein of 21kD, identified as the Bax protein. This monoclonal antibody is highly specific to Bax and shows no cross-reaction with Bcl-2a or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2. Applications: Suitable for use in Flow Cytometry, Immunofluorescence, ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT Flow Cytometry: 0.5-1ug/10e6 cells Western Blot: 0.5-1ug/ml Immunofluorescence: 1-2ug/ml Immunoprecipitation: 1-2ug/500ug protein lysate Optimal dilutions to be determined by the researcher. Positive Control: Jurkat, K562, HL-60, or HeLa Cells. Reed-Sternberg cells in Hodgkin’s lymphoma. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Calc Crossreactivity :
Hu Mk
Immunogen :
Synthetic peptide corresponding to aa3-16 (Cys-GSGEQPRGGGPTSS) from human Bax.
Specificity :
Recognizes human Bax. Species Crossreactivity: monkey. Does not react with mouse or rat.
Purity :
Purified by Protein G affinity chromatography.
Form :
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138495-AF.
Concentration :
~0.2mg/ml
Desc2 :
Product Type: Mab Isotype: IgG1 Clone No: 2D2 Host: mouse Source: human Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138495AF. Purity: Purified by Protein G affinity chromatography. Immunogen: Synthetic peptide corresponding to aa3-16 (Cys-GSGEQPRGGGPTSS) from human Bax. Specificity: Recognizes human Bax. Species Crossreactivity: monkey. Does not react with mouse or rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
E FC IF IHC IP WB
Storage Temperature :
-20°C
Reference :
1. Hsu YT, et. al. Journal of Biological Chemistry, 1997, 272(21):13829-34. 2. Hsu YT, et. al. PNAS, 1997, 94(8):3668-72. 3. Wolter KG, et. al. Journal of Cell Biology, 1997, 139(5):1281-92.
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/30/138495_1.jpg
company information
US Biological
4 Technology Way
Salem, MA01970
service@usbio.net
https://www.usbio.net
800-520-3011
headquarters: USA
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