WT1, ID (E361) (WT1, Wilms tumor protein, WT33) (AP) | US Biological 043900-AP product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

WT1, ID (E361) (WT1, Wilms tumor protein, WT33) (AP)

catalog :

043900-AP

quantity :

200 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

reactivity :

human

product information

Catalog Number :

043900-AP

Product wo Prefix :

WT1, ID (E361) (WT1, Wilms tumor protein, WT33) (AP)

Host :

rabbit

Product Type :

Pab

Conjugate :

Category :

Antibodies

Size1 :

200 ul

Isotype :

IgG

Desc1 :

This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation site upstream of and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq]. Applications: Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Formalin fixed and paraffin embedded tissues Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Calc Crossreactivity :

Hu Mo

Alternate Name :

WT1, Wilms tumor protein, WT33

Immunogen :

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 346-375 in the central region of human WT1. Species Sequence Homology: rat, porcine, Xenopus

Specificity :

Recogizes human WT1. Species Crossreactivity: mouse

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phophatase (AP).

Concentration :

As Reported

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As Reported Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phophatase (AP). Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 346-375 in the central region of human WT1. Species Sequence Homology: rat, porcine, Xenopus Specificity: Recogizes human WT1. Species Crossreactivity: mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IHC WB

Storage Temperature :

4°C Do Not Freeze

Reference :

Sitaram, R.T., et al. Br. J. Cancer 103(8):1255-1262(2010) Dohi, S., et al. Anticancer Res. 30(8):3187-3192(2010) Rocquain, J., et al. BMC Cancer 10, 401 (2010) : Wagner, K.D., et al. J. Cell. Sci. 116 (PT 9), 1653-1658 (2003) : Mitsuya, K., et al. Hum. Mol. Genet. 6(13):2243-2246(1997)

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/16/043900-AP_1.jpg