antibody

WNT5A, ID (WNT5A, Protein Wnt-5a)

043892

200 ul

polyclonal

domestic rabbit

nonconjugated

WNT5A, ID (WNT5A, Protein Wnt-5a)

rabbit

Pab

Antibodies

200 ul

IgG

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98%, 98% and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction. [provided by RefSeq]. Applications: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry and ELISA. Other applications have not been tested. Recommended Dilution: Western Blot: 1:2000 Immunohistochemistry (FFPE): 1:50-1:100 Flow Cytometry: 1:10-1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mo Rt

WNT5A, Protein Wnt-5a

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 185-213 in the central region of human WNT5A, Swiss/UniProt Accession: P41221

Recognizes human WNT5A. Species Crossreactivity: mouse, rat

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

As reported

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As reported Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 185-213 in the central region of human WNT5A, Swiss/UniProt Accession: P41221 Specificity: Recognizes human WNT5A. Species Crossreactivity: mouse, rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E FC IHC WB

-20°C

He, X., et al. Science 275(5306):1652-1654(1997) Danielson, K.G., et al. J. Biol. Chem. 270(52):31225-31234(1995) Smolich, B.D., et al. Mol. Biol. Cell 4(12):1267-1275(1993) Clark, C.C., et al. Genomics 18(2):249-260(1993)

https://usbio-images.r.worldssl.net/prodimages/14/043892_1.jpg

Flow Cytometric analysis of Hela cells using 043892 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

https://usbio-images.r.worldssl.net/prodimages/14/043892_5.png

Overlay histogram showing Hela cells stained with 043892 (green line). The cells were fixed with 2% paraformaldehyde (10min) and then permeabilized with 90% methanol for 10min. The cells were then incubated in 2% BSA to block non-specific protein-protein interactions followed by 043892 (1:25) for 60min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1:200 for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10e6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.