WNT5A, ID (WNT5A, Protein Wnt-5a) (APC) | US Biological 043892-APC product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

WNT5A, ID (WNT5A, Protein Wnt-5a) (APC)

catalog :

043892-APC

quantity :

200 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

APC

reactivity :

human

product information

Catalog Number :

043892-APC

Product wo Prefix :

WNT5A, ID (WNT5A, Protein Wnt-5a) (APC)

Host :

rabbit

Product Type :

Pab

Conjugate :

APC

Category :

Antibodies

Size1 :

200 ul

Isotype :

IgG

Desc1 :

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98%, 98% and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction. [provided by RefSeq]. Applications: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry and FLISA. Other applications have not been tested. Recommended Dilution: Immunohistochemistry: Formalin fixed, paraffin-embedded sections Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Calc Crossreactivity :

Hu Mo Rt

Alternate Name :

WNT5A, Protein Wnt-5a

Immunogen :

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 185-213 in the central region of human WNT5A, Swiss/UniProt Accession: P41221

Specificity :

Recognizes human WNT5A. Species Crossreactivity: mouse, rat

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

Concentration :

As reported

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As reported Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC). Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 185-213 in the central region of human WNT5A, Swiss/UniProt Accession: P41221 Specificity: Recognizes human WNT5A. Species Crossreactivity: mouse, rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

FC FL IHC WB

Storage Temperature :

4°C Do Not Freeze

Reference :

He, X., et al. Science 275(5306):1652-1654(1997) Danielson, K.G., et al. J. Biol. Chem. 270(52):31225-31234(1995) Smolich, B.D., et al. Mol. Biol. Cell 4(12):1267-1275(1993) Clark, C.C., et al. Genomics 18(2):249-260(1993)

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/39/043892-APC_1.jpg

Picture4 Text :

Flow Cytometric analysis of Hela cells using 043892 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Picture 5 File Name :

https://usbio-images.r.worldssl.net/prodimages/39/043892-APC_5.png

Picture5 Text :

Overlay histogram showing Hela cells stained with 043892 (green line). The cells were fixed with 2% paraformaldehyde (10min) and then permeabilized with 90% methanol for 10min. The cells were then incubated in 2% BSA to block non-specific protein-protein interactions followed by 043892 (1:25) for 60min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1:200 for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10e6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.