antibody

USP7, ID (USP7, HAUSP, Ubiquitin Carboxyl-terminal Hydrolase 7, Deubiquitinating Enzyme 7, Herpes Virus-associated Ubiquitin-specific Protease, Ubiquitin Thioesterase 7, Ubiquitin-specific-processing Protease 7) (AP)

043678-AP

200 ul

polyclonal

domestic rabbit

AP

USP7, ID (USP7, HAUSP, Ubiquitin Carboxyl-terminal Hydrolase 7, Deubiquitinating Enzyme 7, Herpes Virus-associated Ubiquitin-specific Protease, Ubiquitin Thioesterase 7, Ubiquitin-specific-processing Protease 7) (AP)

rabbit

Pab

AP

Antibodies

200 ul

IgG

Hydrolase that deubiquitinates target proteins such as FOXO4, TP53, MDM2, PTEN and DAXX. Together with DAXX, prevents MDM2 self-ubiquitination and enhances the E3 ligase activity of MDM2 towards TP53, thereby promoting TP53 ubiquitination and proteasomal degradation. Deubiquitinates TP53 and MDM2 and strongly stabilizes TP53 even in the presence of excess MDM2, and also induces TP53-dependent cell growth repression and apoptosis. Deubiquitination of FOXO4 in presence of hydrogen peroxide is not dependent on TP53 and inhibits FOXO4-induced transcriptional activity. In association with DAXX, is involved in the deubiquitination and translocation of PTEN from the nucleus to the cytoplasm, both processes that are counteracted by PML. Involved in cell proliferation during early embryonic development. Contributes to the overall stabilization and trans-activation capability of the Herpes virus 1 trans-acting transcriptional protein ICP0/VMW110 during HSV-1 infection. Applications: Suitable for use in Western Blot and ELISA. Other applications have not been tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Hu

USP7, HAUSP, Ubiquitin carboxyl-terminal hydrolase 7, Deubiquitinating enzyme 7, Herpesvirus-associated ubiquitin-specific protease, Ubiquitin thioesterase 7, Ubiquitin-specific-processing protease 7

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 320-348 in the central region of human USP7. Species sequence homology: rat

Recognizes human USP7.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phophatase (AP).

As reported

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As reported Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phophatase (AP). Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 320-348 in the central region of human USP7. Species sequence homology: rat Specificity: Recognizes human USP7. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E WB

4°C Do Not Freeze

1. Sarkari, F., et al., J. Mol. Biol. 402(5): 825-837 (2010). 2. de Bie, P., et al., Biochem. Biophys. Res. Commun. 400(3): 389-395 (2010). 3. Maertens, G.N., et al., EMBO J. 29(15): 2553-2565 (2010). 4. Rose, J.E., et al., Mol. Med. 16(7-8): 247-253 (2010). 5. Tang, J., et al., Biochem. Biophys. Res. Commun. 393(3): 542-545 (2010).

https://usbio-images.r.worldssl.net/prodimages/37/043678-AP_1.jpg