antibody

SUPT5H, ID (SUPT5H, SPT5, SPT5H, Transcription elongation factor SPT5, DRB sensitivity-inducing factor 160kD subunit, DRB sensitivity-inducing factor large subunit, Tat-cotransactivator 1 protein) (APC)

042487-APC

200 ul

polyclonal

domestic rabbit

APC

SUPT5H, ID (SUPT5H, SPT5, SPT5H, Transcription elongation factor SPT5, DRB sensitivity-inducing factor 160kD subunit, DRB sensitivity-inducing factor large subunit, Tat-cotransactivator 1 protein) (APC)

rabbit

Pab

APC

Antibodies

200 ul

IgG

Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences. Applications: Suitable for use in Western Blot, FLISA Recommended Dilution: FLISA: 1:1,000 Western Blot: 1:100-500 Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Hu

SUPT5H, SPT5, SPT5H, Transcription elongation factor SPT5, DRB sensitivity-inducing factor 160kD subunit, DRB sensitivity-inducing factor large subunit, Tat-cotransactivator 1 protein

SUPT5H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 394-424 amino acids from the Central region of human SUPT5H.

Human

Purified by Protein A affinity chromatography.

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

As reported

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As reported Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC). Purity: Purified by Protein A affinity chromatography. Immunogen: SUPT5H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 394-424 amino acids from the Central region of human SUPT5H. Specificity: Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

FL WB

4°C Do Not Freeze

Wenzel, S., et al. Biochem. J. 425(2):373-380(2010) Chen, Y., et al. Genes Dev. 23(23):2765-2777(2009) Komori, T., et al. Genes Cells 14(3):343-354(2009) Chen, H., et al. PLoS ONE 4 (9), E6918 (2009) : Amir-Zilberstein, L., et al. J. Biol. Chem. 283(3):1317-1323(2008)

https://usbio-images.r.worldssl.net/prodimages/45/042487-APC_1.jpg