SMAD3 (S208) (SMAD3, MADH3, Mothers against decapentaplegic homolog 3, JV15-2, SMAD family member 3)

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

SMAD3 (S208) (SMAD3, MADH3, Mothers against decapentaplegic homolog 3, JV15-2, SMAD family member 3)

catalog :

042021

quantity :

200 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

042021

Product wo Prefix :

SMAD3 (S208) (SMAD3, MADH3, Mothers against decapentaplegic homolog 3, JV15-2, SMAD family member 3)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

200 ul

Isotype :

IgG

Desc1 :

SMAD3 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:10-1:50 Immunofluorescence: 1:10-1:50 Flow Cytometry: 1:10-1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mo

Alternate Name :

SMAD3, MADH3, Mothers against decapentaplegic homolog 3, JV15-2, SMAD family member 3

Immunogen :

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 186-215 of human SMAD3, UniProt Accession #P84022. Species Sequence Homology: chicken, porcine and rat

Specificity :

Recognizes human SMAD3. Species Crossreactivity: mouse

Purity :

Purified by Protein A affinity chromatography

Form :

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide

Concentration :

As Reported

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As Reported Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide Purity: Purified by Protein A affinity chromatography Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 186-215 of human SMAD3, UniProt Accession #P84022. Species Sequence Homology: chicken, porcine and rat Specificity: Recognizes human SMAD3. Species Crossreactivity: mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E FC IF IHC WB

Storage Temperature :

-20°C

Reference :

1. Zhang, M., et al. J. Biol. Chem. 285(12):8703-8710(2010). 2. Daly, A.C., et al. J. Biol. Chem. 285(9):6489-6497(2010). 3. Heikkinen, P.T., et al. J. Biol. Chem. 285(6):3740-3749(2010). 4. Tseng, Z.H., et al. Heart Rhythm 6(12):1745-1750(2009)

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/38/042021_1.jpg

Picture4 Text :

Flow cytometric analysis of 042021 using MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.