antibody

Siglec 10, CT (Siglec 10, SLG2, Sialic acid-binding Ig-like lectin 10, Siglec like protein 2) (AP)

041732-AP

200 ul

polyclonal

domestic rabbit

AP

Siglec 10, CT (Siglec 10, SLG2, Sialic acid-binding Ig-like lectin 10, Siglec like protein 2) (AP)

rabbit

Pab

AP

Antibodies

200 ul

IgG

SIGLEC10 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It preferentially binds to alpha2,3- or 2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. SIGLEC10 interacts with PTPN6/SHP-1 upon phosphorylation. The protein is expressed by peripheral blood leukocytes (eosinophils, monocytes and a natural killer cell subpopulation). Isoform 5 is found to be the most abundant isoform, found in lymph node, lung, ovary and appendix. Isoform 1 is found at high levels and isoform 2 at lower levels in bone marrow, spleen and spinal chord. Isoform 2 is also found in brain. Isoform 4 is specifically found in natural killer cells. SIGLEC10 contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in downmodulation of cellular responses. The phosphorylated ITIM motif binds to the SH2 domain of PTPN6/SHP-1. Phosphorylation of Tyr-667 is involved in binding to PTPN6. The SIGLEC10 gene belongs to the immunoglobulin superfamily. Applications: Suitable for use in Western Blot, ELISA Recommended Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Storage and Stability: Store product at 4°C. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Hu

SIGLEC10, SLG2, Sialic acid-binding Ig-like lectin 10, Siglec-like protein 2

SIGLEC10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human SIGLEC10.

Human

Purified by Protein A affinity chromatography.

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phophatase (AP).

As reported

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As reported Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phophatase (AP). Purity: Purified by Protein A affinity chromatography. Immunogen: SIGLEC10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human SIGLEC10. Specificity: Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E WB

4°C Do Not Freeze

Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002). Kitzig, F., et al., Biochem. Biophys. Res. Commun. 296(2):355-362 (2002). Li, N., et al., J. Biol. Chem. 276(30):28106-28112 (2001). Yousef, G.M., et al., Biochem. Biophys. Res. Commun. 284(4):900-910 (2001). Munday, J., et al., Biochem. J. 355 (Pt 2), 489-497 (2001).

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