antibody

SFTPC, CT (SFTPC, SFTP2, Pulmonary surfactant-associated protein C, Pulmonary surfactant-associated proteolipid SPL(Val), SP5)

041641

200 ul

polyclonal

domestic rabbit

nonconjugated

SFTPC, CT (SFTPC, SFTP2, Pulmonary surfactant-associated protein C, Pulmonary surfactant-associated proteolipid SPL(Val), SP5)

rabbit

Pab

Antibodies

200 ul

IgG

This gene encodes the pulmonary-associated surfactant protein C (SPC), an extremely hydrophobic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 2, also called pulmonary alveolar proteinosis due to surfactant protein C deficiency, and are associated with interstitial lung disease in older infants, children, and adults. Alternatively spliced transcript variants encoding different protein isoforms have been identified. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:10-1:50 Immunofluorescence : 1:10-1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

SFTPC, SFTP2, Pulmonary surfactant-associated protein C, Pulmonary surfactant-associated proteolipid SPL(Val), SP5

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 144-173 in the C-terminal region of human SFTPC, UniProt Accession #NP_001165828.1; P11686.

Recognizes human SFTPC.

Purified by Protein A affinity chromatography.

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

As Reported

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As Reported Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Purity: Purified by Protein A affinity chromatography. Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 144-173 in the C-terminal region of human SFTPC, UniProt Accession #NP_001165828.1; P11686. Specificity: Recognizes human SFTPC. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E IF IHC WB

-20°C

1. Wambach, J.A., et al. Pediatr. Res. 68(3):216-220(2010) 2. Schuurhof, A., et al. Pediatr. Pulmonol. 45(6):608-613(2010) 3. Thouvenin, G., et al. Arch. Dis. Child. 95(6):449-454(2010) 4. Crossno, P.F., et al. Chest 137(4):969-973(2010) 5. Davila, S., et al. Genes Immun. 11(3):232-238(2010)

https://usbio-images.r.worldssl.net/prodimages/19/041641_1.jpg

Confocal immunofluorescent analysis with HepG2 cells using 041641 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).