antibody

SERPINI1, NT (SERPINI1, PI12, Neuroserpin, Peptidase inhibitor 12, Serpin I1)

041591

200 ul

polyclonal

domestic rabbit

nonconjugated

SERPINI1, NT (SERPINI1, PI12, Neuroserpin, Peptidase inhibitor 12, Serpin I1)

rabbit

Pab

Antibodies

200 ul

IgG

This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The protein is primarily secreted by axons in the brain, and preferentially reacts with and inhibits tissue-type plasminogen activator. It is thought to play a role in the regulation of axonal growth and the development of synaptic plasticity. Mutations in this gene result in familial encephalopathy with neuroserpin inclusion bodies (FENIB), which is a dominantly inherited form of familial encephalopathy and epilepsy characterized by the accumulation of mutant neuroserpin polymers. Multiple alternatively spliced variants, encoding the same protein, have been identified. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry and ELISA. Other applications have not been tested. Recommended Dilution: Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:50-1:100 Immunofluorescence: 1:10-1:50 Flow Cytometry: 1:10-1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

SERPINI1, PI12, Neuroserpin, Peptidase inhibitor 12, Serpin I1

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 19-45 in the N-terminal region of human SERPINI1.

Recognizes human SERPINI1.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

As reported

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As reported Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 19-45 in the N-terminal region of human SERPINI1. Specificity: Recognizes human SERPINI1. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E FC IF IHC WB

-20°C

1. Takehara, S., et al. J. Mol. Biol. 403(5):751-762(2010) 2. Han, S., et al. Hum. Immunol. 71(7):727-730(2010) 3. Rajaraman, P., et al. Cancer Epidemiol. Biomarkers Prev. 9(5):1356-1361(2010) 4. Davies, M.J., et al. J. Biol. Chem. 284(27):18202-18209(2009) 5. Rajaraman, P., et al. Cancer Epidemiol. Biomarkers Prev. 18(5):1651-1658(2009)

https://usbio-images.r.worldssl.net/prodimages/40/041591_1.png

Confocal immunofluorescent analysis of HepG2 cells using 041591 followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

https://usbio-images.r.worldssl.net/prodimages/40/041591_5.png

Flow Cytometric analysis of HepG2 cells using 041591 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.