antibody

RFWD2, CT (RFWD2, COP1, RNF200, E3 ubiquitin-protein ligase RFWD2, Constitutive photomorphogenesis protein 1 homolog, RING finger and WD repeat domain protein 2, RING finger protein 200) (AP)

040984-AP

200 ul

polyclonal

domestic rabbit

AP

RFWD2, CT (RFWD2, COP1, RNF200, E3 ubiquitin-protein ligase RFWD2, Constitutive photomorphogenesis protein 1 homolog, RING finger and WD repeat domain protein 2, RING finger protein 200) (AP)

rabbit

Pab

AP

Antibodies

200 ul

IgG

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Applications: Suitable for use in Western Blot, ELISA Recommended Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Storage and Stability: Store product at 4°C. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Hu

RFWD2, COP1, RNF200, E3 ubiquitin-protein ligase RFWD2, Constitutive photomorphogenesis protein 1 homolog, RING finger and WD repeat domain protein 2, RING finger protein 200

RFWD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 672-701 amino acids from the C-terminal region of human RFWD2.

Human

Purified by Protein A affinity chromatography.

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phophatase (AP).

As reported

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As reported Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phophatase (AP). Purity: Purified by Protein A affinity chromatography. Immunogen: RFWD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 672-701 amino acids from the C-terminal region of human RFWD2. Specificity: Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E WB

4°C Do Not Freeze

Shimada, M., et al. Hum. Genet. 128(4):433-441(2010) Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) : Kinyo, A., et al. J. Invest. Dermatol. 130(2):541-545(2010) Li, D.Q., et al. Proc. Natl. Acad. Sci. U.S.A. 106(41):17493-17498(2009) Kato, S., et al. J. Biol. Chem. 283(51):35464-35473(2008)

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