antibody

POLG, CT (POLG, MDP1, POLG1, POLGA, DNA polymerase subunit gamma-1, Mitochondrial DNA polymerase catalytic subunit, PolG-alpha) (APC)

040244-APC

200 ul

polyclonal

domestic rabbit

APC

POLG, CT (POLG, MDP1, POLG1, POLGA, DNA polymerase subunit gamma-1, Mitochondrial DNA polymerase catalytic subunit, PolG-alpha) (APC)

rabbit

Pab

APC

Antibodies

200 ul

IgG

Mitochondrial DNA polymerase is heterotrimeric, consisting of a homodimer of accessory subunits plus a catalytic subunit. The protein encoded by this gene is the catalytic subunit of mitochondrial DNA polymerase. The encoded protein contains a polyglutamine tract near its N-terminus that may be polymorphic. Defects in this gene are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions 1 (PEOA1), sensory ataxic neuropathy dysarthria and ophthalmoparesis (SANDO), Alpers-Huttenlocher syndrome (AHS), and mitochondrial neurogastrointestinal encephalopathy syndrome (MNGIE). Two transcript variants encoding the same protein have been found for this gene. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, FLISA Recommended Dilution: FLISA: 1:1,000 Western Blot: 1:100-500 Flow Cytometry: 1:10-50 Immunofluorescence: 1:10-50 Immunohistochemistry: 1:10-50 Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Hu Mo

POLG, MDP1, POLG1, POLGA, DNA polymerase subunit gamma-1, Mitochondrial DNA polymerase catalytic subunit, PolG-alpha

KLH-conjugated synthetic peptide mapping to a fragment of residues within aa1119-1148 from the C-terminal region of human POLG. Species sequence homology: mouse

Recognizes human POLG.

Purified by Protein A affinity chromatography.

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

As reported

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As reported Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC). Purity: Purified by Protein A affinity chromatography. Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within aa1119-1148 from the C-terminal region of human POLG. Species sequence homology: mouse Specificity: Recognizes human POLG. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

FC FL IF IHC WB

4°C Do Not Freeze

Tong, Z.B., et al. Fertil. Steril. 94(7):2932-2934(2010) Stewart, J.D., et al. Hepatology 52(5):1791-1796(2010) Batabyal, D., et al. J. Biol. Chem. 285(44):34191-34201(2010) Wang, W., et al. Nucleic Acids Res. (2010) In press : Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010)

https://usbio-images.r.worldssl.net/prodimages/43/040244-APC_1.jpg

POLG Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POLG Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.