This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
US Biological
product type :
antibody
product name :
PGK1, ID (PGK1, PGKA, Phosphoglycerate kinase 1, Cell migration-inducing gene 10 protein, Primer recognition protein 2)
catalog :
039920
quantity :
200 ul
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
product information
Catalog Number :
039920
Product wo Prefix :
PGK1, ID (PGK1, PGKA, Phosphoglycerate kinase 1, Cell migration-inducing gene 10 protein, Primer recognition protein 2)
Host :
rabbit
Product Type :
Pab
Category :
Antibodies
Size1 :
200 ul
Isotype :
IgG
Desc1 :
Also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), this major enzyme in glycolysis catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, generating one molecule of ATP. New blood vessel formation or angiogenesis is critical for tumor expansion and metastasis. Lay et al. (2000) showed that the plasmin reductase isolated from conditioned medium of fibrosarcoma cells is the glycolytic enzyme phosphoglycerate kinase. They concluded that phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and participates in the angiogenic process as a disulfide reductase.
Applications:
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA
Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow cytometry
Immunofluorescence: 1:10-50
Flow Cytometry: 1:10-50
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Calc Crossreactivity :
Hu Mo
Alternate Name :
PGK1, PGKA, Phosphoglycerate kinase 1, Cell migration-inducing gene 10 protein, Primer recognition protein 2
Immunogen :
PGK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124~153 amino acids from the center region of human PGK1.
Specificity :
Human, mouse
Purity :
Purified by Protein G affinity chromatography.
Form :
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Concentration :
As reported
Desc2 :
Product Type: Pab
Isotype: IgG
Host: rabbit
Source: human
Concentration: As reported
Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity: Purified by Protein G affinity chromatography.
Immunogen: PGK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124~153 amino acids from the center region of human PGK1.
Specificity: Human, mouse
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
E FC IF IHC WB
Storage Temperature :
-20°C
Reference :
Lay, A. J., et al. Nature 408: 869-873 (2000).
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/37/039920_1.jpg
Picture4 Text :
Confocal immunofluorescent analysis of PGK1 Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Picture 5 File Name :
https://usbio-images.r.worldssl.net/prodimages/37/039920_5.jpg
Picture5 Text :
Confocal immunofluorescent analysis of PGK1 Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
company information
US Biological
4 Technology Way
Salem, MA01970
Salem, MA01970
service@usbio.net
https://www.usbio.net800-520-3011
headquarters: USA
United States Biological is a primary brand for the Life Science Industry. We are committed to reducing the cost of research with value, integrity and a truly personal buying experience.
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