antibody

P21Cip1 (S130) (CDKN1A, CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1, Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein 6, p21)

039563

200 ul

polyclonal

domestic rabbit

nonconjugated

P21Cip1 (S130) (CDKN1A, CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1, Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein 6, p21)

rabbit

Pab

Antibodies

200 ul

IgG

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported. Applications: Suitable for use in Western Blot, Immunofluorescence, ELISA Recommended Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunofluorescence: 1:10-50 Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

CDKN1A, CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1, Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein 6, p21

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 105-140 from the S130 region of human P21Cip1.

Recognizes human P21Cip1.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

As reported

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As reported Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 105-140 from the S130 region of human P21Cip1. Specificity: Recognizes human P21Cip1. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E IF WB

-20°C

1. Scott, S.A., et al., Leuk. Res. 28(12):1293-1301 (2004). 2. Amini, S., et al., J. Biol. Chem. 279(44):46046-46056 (2004). 3. Chen, T., et al., Cancer Res. 64(20):7412-7419 (2004). 4. Sieburg, M., et al., J. Virol. 78(19):10399-10409 (2004). 5. Giraud, S., et al., Oncogene 23(44):7391-7398 (2004).

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