NANOG, ID (NANOG, Homeobox protein NANOG, Homeobox transcription factor Nanog)

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

NANOG, ID (NANOG, Homeobox protein NANOG, Homeobox transcription factor Nanog)

catalog :

038783

quantity :

200 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

038783

Product wo Prefix :

NANOG, ID (NANOG, Homeobox protein NANOG, Homeobox transcription factor Nanog)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

200 ul

Isotype :

IgG

Desc1 :

NANOG is a Ttranscription regulator involved in inner cell mass and embryonic stem (ES) cels proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA Recommended Dilution: ELISA: 1:1,000 Western Blot: 1:50-100 Immunohistochemistry: 1:10-50 Immunofluorescence: 1:10-50 Flow Cytometry: 1:10-50 Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Alternate Name :

NANOG, Homeobox protein NANOG, Homeobox transcription factor Nanog

Immunogen :

NANOG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 101~131 amino acids from the center region of human NANOG.

Specificity :

Human

Purity :

Purified by Protein A affinity chromatography.

Form :

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Concentration :

As reported

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As reported Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Purity: Purified by Protein A affinity chromatography. Immunogen: NANOG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 101~131 amino acids from the center region of human NANOG. Specificity: Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E FC IF IHC WB

Storage Temperature :

-20°C

Reference :

Kochupurakkal,B.S., Biochem. Biophys. Res. Commun. 365 (4), 846-850 (2008) Freberg,C.T., Mol. Biol. Cell 18 (5), 1543-1553 (2007)

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/24/038783_1.jpg

Picture4 Text :

Flow cytometric analysis of hela cells using NANOG Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Picture5 Text :