antibody

LSD1, CT (KDM1A, AOF2, KDM1, KIAA0601, LSD1, Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2)

037992

200 ul

polyclonal

domestic rabbit

nonconjugated

LSD1, CT (KDM1A, AOF2, KDM1, KIAA0601, LSD1, Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2)

rabbit

Pab

Antibodies

200 ul

IgG

LSD1 is a histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. LSD1 contains a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. LSD1 demethylates both mono- and tri-methylted 'Lys-4' of histone H3. This protein may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. It may also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunohistochemistry: 1:50-100 Immunofluorescence: 1:10-1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

KDM1A, AOF2, KDM1, KIAA0601, LSD1, Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 819-852 in the C-terminal region of human LSD1, UniProt Accession #O60341. Species Sequence Homology: Mouse

Recognizes human LSD1.

Purified by ammonium sulfate precipitation.

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

As reported

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As reported Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Purity: Purified by ammonium sulfate precipitation. Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 819-852 in the C-terminal region of human LSD1, UniProt Accession #O60341. Species Sequence Homology: Mouse Specificity: Recognizes human LSD1. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E IF IHC WB

-20°C

1. Forneris,F., et al. FEBS Lett. 579 (10), 2203-2207 (2005) 2. Shi,Y., et al. Cell 119 (7), 941-953 (2004) 3. Hakimi,M.A., et al. J. Biol. Chem. 278 (9), 7234-7239 (2003) 4. Hakimi,M.A., et al. PNAS 99 (11), 7420-7425 (2002) 5. Humphrey,G.W., et al. J. Biol. Chem. 276 (9), 6817-6824 (2001) 6. Ota, T., et al., Nat. Genet. 36(1):40-45 (2004).

https://usbio-images.r.worldssl.net/prodimages/28/037992_1.png

Confocal immunofluorescent analysis of 293 cells using 037992 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).