antibody

KRAS, CT (KRAS, KRAS2, RASK2, GTPase KRas, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras, GTPase KRas, N-terminally processed)

037592

200 ul

polyclonal

domestic rabbit

nonconjugated

KRAS, CT (KRAS, KRAS2, RASK2, GTPase KRas, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras, GTPase KRas, N-terminally processed)

rabbit

Pab

Antibodies

200 ul

IgG

This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. Applications: Suitable for use in Western Blot, Immunofluorescence, Flow Cytometry and ELISA. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:500-1:2000 Immunofluorescence: 1:10-1:50 Flow Cytometry: 1:10-1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mo Rt

KRAS, KRAS2, RASK2, GTPase KRas, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras, GTPase KRas, N-terminally processed

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 146-174 near the C-terminal region of human KRAS.

Recognizes human KRAS. Species Crossreactivity: mouse and rat

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

As Reported

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As Reported Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 146-174 near the C-terminal region of human KRAS. Specificity: Recognizes human KRAS. Species Crossreactivity: mouse and rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E FC IF WB

-20°C

1. Bruckman, K.C., et al. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 110(5):632-637(2010). 2. Wong, K.K., et al. Am. J. Pathol. 177(4):1611-1617(2010). 3. Irahara, N., et al. Diagn. Mol. Pathol. 19(3):157-163(2010). 4. Carotenuto, P., et al. Pharmacogenomics 11(8):1169-1179(2010). 5. Leventopoulos, G., et al. Clin. Exp. Rheumatol. 28(4):556-557(2010)

https://usbio-images.r.worldssl.net/prodimages/12/037592_1.jpg

Confocal immunofluorescent analysis of WiDr cells using 037592 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

https://usbio-images.r.worldssl.net/prodimages/12/037592_5.jpg

Flow Cytometric analysis of Hela cells using 037592 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.