antibody

IL6, ID (IL6, IFNB2, Interleukin-6, B Cell stimulatory factor 2, CTL differentiation factor, Hybridoma growth factor, Interferon beta-2)

037016

200 ul

polyclonal

domestic rabbit

nonconjugated

IL6, ID (IL6, IFNB2, Interleukin-6, B Cell stimulatory factor 2, CTL differentiation factor, Hybridoma growth factor, Interferon beta-2)

rabbit

Pab

Antibodies

200 ul

IgG

This gene encodes a cytokine that functions in inflammation and the maturation of B cells. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Applications: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry and ELISA. Other applications have not been tested. Recommended Dilution: Western Blot: 1:1000-1:2000 Flow Cytometry: 1:10-1:50 Immunohistochemistry (FFPE): 1:10-1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

IL6, IFNB2, Interleukin-6, B-cell stimulatory factor 2, CTL differentiation factor, Hybridoma growth factor, Interferon beta-2

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 27-55 in the central region of human IL6, UniProt Accession #P05231.

Recognizes human IL6.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

As reported

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As reported Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 27-55 in the central region of human IL6, UniProt Accession #P05231. Specificity: Recognizes human IL6. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E FC IHC WB

-20°C

1. Perez, A.B., et al. Hum. Immunol. 71(11):1135-1140(2010) 2. Weng, X.M., et al. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 24(1):42-44(2010) 3. Santhosh, S., et al. Trop Gastroenterol 31(1):30-33(2010) 4. Gein, O.N., et al. Patol Fiziol Eksp Ter 1, 10-13 (2010) : 5. Tuttolomondo, A., et al. Cardiovasc Diabetol 9, 50 (2010) :

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Immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue using 037016 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of 037016 for immunohistochemistry.

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Flow Cytometric analysis of NCI-H460 cells using 037016 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.