antibody

HP, ID (HP, Haptoglobin, Zonulin, Haptoglobin alpha chain, Haptoglobin beta chain)

036741

200 ul

polyclonal

domestic rabbit

nonconjugated

HP, ID (HP, Haptoglobin, Zonulin, Haptoglobin alpha chain, Haptoglobin beta chain)

rabbit

Pab

Antibodies

200 ul

IgG

This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry and ELISA. Other applications have not been tested. Recommended Dilution: Western Blot: 1:1000-1:2000 Immunohistochemistry (FFPE): 1:50-1:100 Flow Cytometry: 1:10-1:50 Immunofluorescence: 1:10-1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mo

HP, Haptoglobin, Zonulin, Haptoglobin alpha chain, Haptoglobin beta chain

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 296-322 in the central region of human HP.

Recognizes human HP. Species crossreactivity: mouse

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

As reported

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As reported Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 296-322 in the central region of human HP. Specificity: Recognizes human HP. Species crossreactivity: mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E FC IF IHC WB

-20°C

1. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) 2. Ucisik-Akkaya, E., et al. Mol. Hum. Reprod. 16(10):770-777(2010) 3. Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010) 4. Savy, M., et al. PLoS ONE 5 (6), E11075 (2010) : 5. Kasvosve, I., et al. Adv Clin Chem 50, 23-46 (2010) :

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Flow Cytometric analysis of MDA-MB435 cells using 036741 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

https://usbio-images.r.worldssl.net/prodimages/36/036741_5.png

Confocal immunofluorescent analysis of MDA-MB435 cells using 036741 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).