antibody

HLA-DQA1, NT (HLA-DQA1, HLA class II histocompatibility antigen, DQ alpha 1 chain, DC-1 alpha chain, DC-alpha, HLA-DCA, MHC class II DQA1)

036623

200 ul

polyclonal

domestic rabbit

nonconjugated

HLA-DQA1, NT (HLA-DQA1, HLA class II histocompatibility antigen, DQ alpha 1 chain, DC-1 alpha chain, DC-alpha, HLA-DCA, MHC class II DQA1)

rabbit

Pab

Antibodies

200 ul

IgG

HLA-DQA1 belongs to the HLA class II alpha chain paralogues. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. Applications: Suitable for use in ELISA, Western Blot and Flow Cytometry. Other applications have not tested. Recommended Dilutions: Western Blot: 1:1000 Flow Cytometry: 1:10-1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

HLA-DQA1, HLA class II histocompatibility antigen, DQ alpha 1 chain, DC-1 alpha chain, DC-alpha, HLA-DCA, MHC class II DQA1

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 39-66 in the N-terminal region of human HLA-DQA1.

Recognizes human HLA-DQA1.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

As Reported

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As Reported Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 39-66 in the N-terminal region of human HLA-DQA1. Specificity: Recognizes human HLA-DQA1. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E FC WB

-20°C

1. Donaldson, P.T., et al. J. Hepatol. 53(6):1049-1053(2010) 2. Noble, J.A., et al. Diabetes 59(11):2972-2979(2010) 3. Ferreira, R.C., et al. Nat. Genet. 42(9):777-780(2010) 4. Ovsyannikova, I.G., et al. PLoS ONE 5 (7), E11806 (2010) 5. Xun, Y.H., et al. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 23(6):430-433(2009)

https://usbio-images.r.worldssl.net/prodimages/34/036623_1.png

Flow Cytometric analysis of NCI-H460 cells using 036623 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.