antibody

FGFR4, NT (FGFR4, JTK2, TKF, Fibroblast growth factor receptor 4, CD334) (AP)

035643-AP

200 ul

polyclonal

domestic rabbit

AP

FGFR4, NT (FGFR4, JTK2, TKF, Fibroblast growth factor receptor 4, CD334) (AP)

rabbit

Pab

AP

Antibodies

200 ul

IgG

FGFR4 is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. The genomic organization of the gene, compared to members 1-3, encompasses 18 exons rather than 19 or 20. Although alternative splicing has been observed, there is no evidence that the C-terminal half of the IgIII domain of this protein varies between three alternate forms, as indicated for members 1-3. This particular family member preferentially binds acidic fibroblast growth factor and, although its specific function is unknown, it is overexpressed in gynecological tumor samples, suggesting a role in breast and ovarian tumorigenesis. Applications: Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Formalin-fixed, paraffin-embedded sections Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Hu Mo

FGFR4, JTK2, TKF, Fibroblast growth factor receptor 4, CD334

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 24-55 in the N-terminal region of human FGFR4.

Recognizes human FGFR4 at ~88kD. Species Crossreactivity: mouse

Purified by Protein A/G affinity chromatography

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phophatase (AP).

As reported

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As reported Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phophatase (AP). Purity: Purified by Protein A affinity chromatography Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 24-55 in the N-terminal region of human FGFR4. Specificity: Recognizes human FGFR4 at ~88kD. Species Crossreactivity: mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E IHC WB

4°C Do Not Freeze

1. Jezequel, P., et al., Br. J. Cancer 90(1):189-193 (2004). 2. Qian, Z.R., et al., J. Clin. Endocrinol. Metab. 89(4):1904-1911 (2004). 3. Yu, S., et al., J. Biol. Chem. 278(22):19597-19602 (2003). 4. Becker, N., et al., Cancer Epidemiol. Biomarkers Prev. 12(6):582-583 (2003). 5. Takenaka, H., et al., Arch. Dermatol. Res. 294(7):331-338 (2002).

https://usbio-images.r.worldssl.net/prodimages/46/035643-AP_1.jpg

Immunohistochemistry analysis of formalin-fixed paraffin-embedded human cancer tissue using 035643, followed by peroxidase-conjugated to the secondary antibody and AEC staining.