FGFR2, NT (FGFR2, BEK, KGFR, KSAM, Fibroblast growth factor receptor 2, K-sam, Keratinocyte growth factor receptor, CD332) (AP) | US Biological 035641-AP product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

FGFR2, NT (FGFR2, BEK, KGFR, KSAM, Fibroblast growth factor receptor 2, K-sam, Keratinocyte growth factor receptor, CD332) (AP)

catalog :

035641-AP

quantity :

200 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

reactivity :

human

product information

Catalog Number :

035641-AP

Product wo Prefix :

FGFR2, NT (FGFR2, BEK, KGFR, KSAM, Fibroblast growth factor receptor 2, K-sam, Keratinocyte growth factor receptor, CD332) (AP)

Host :

rabbit

Product Type :

Pab

Conjugate :

Category :

Antibodies

Size1 :

200 ul

Isotype :

IgG

Desc1 :

FGFR2 is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in the gene are associated with many craniosynostotic syndromes and bone malformations. The genomic organization of the gene encompasses 20 exons. Alternative splicing in multiple exons, including those encoding the Ig-like domains, the transmembrane region and the carboxyl terminus, results in varied isoforms which differ in structure and specificity. Isoform 1 has equal affinity for aFGF and bFGF but does not bind KGF. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA Recommended Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 1:10-50 Immunofluorescence: 1:10-50 Storage and Stability: Store product at 4°C. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Calc Crossreactivity :

Hu Mk Mo Rt

Alternate Name :

FGFR2, BEK, KGFR, KSAM, Fibroblast growth factor receptor 2, K-sam, Keratinocyte growth factor receptor, CD332

Immunogen :

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 22-51 in the N-terminal region of human FGFR2, UniProt #P21802.

Specificity :

Recognizes human FGFR2. Species crossreactivity: monkey, mouse, rat

Purity :

Purified by Protein G affinity chromatography.

Form :

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phophatase (AP).

Concentration :

As reported

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As reported Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phophatase (AP). Purity: Purified by Protein G affinity chromatography. Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 22-51 in the N-terminal region of human FGFR2, UniProt #P21802. Specificity: Recognizes human FGFR2. Species crossreactivity: monkey, mouse, rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IF IHC WB

Storage Temperature :

4°C Do Not Freeze

Reference :

Freeman, K.W., et al., Cancer Res. 63(19):6237-6243 (2003). Goriely, A., et al., Science 301(5633):643-646 (2003). Fomenkov, A., et al., J. Biol. Chem. 278(26):23906-23914 (2003). Katoh, M., et al., Int. J. Mol. Med. 11(5):579-583 (2003). Katoh, M., et al., Int. J. Oncol. 22(5):1155-1159 (2003).

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/34/035641-AP_1.jpg

Picture4 Text :

IC staining of Hela cells using 035641. Alexa Fluor488-conjugated goat anti-rabbit lgG (green) was used as secondary antibody. DAPI was used to stain the cell nuclear (blue).

Picture 5 File Name :

https://usbio-images.r.worldssl.net/prodimages/34/035641-AP_5.jpg

Picture5 Text :

Flow Cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibody was used for the analysis.

Picture 6 File Name :

https://usbio-images.r.worldssl.net/prodimages/34/035641-AP_6.jpg

Picture6 Text :

C:FGFR2/isolectinB4 (C) and FGFR1/isolectinB4 (D) staining of apparent mesenchymal cells and the subpopulation of endothelial cells. Virtually all other dispersed apparent mesenchymal cells express FGFR1 and FGFR2 (merged image in E). F: FGFR2 (F) and FGFR1 (G) staining in clustered cells of epithelial origin (inferred by morphology here) demonstrating that epithelial cells express both FGFR1 and FGFR2 (merged image with DAPI staining in H).