antibody

FGFR1, NT (FGFR1, BFGFR, CEK, FGFBR, FLG, FLT2, HBGFR, Fibroblast growth factor receptor 1, Basic fibroblast growth factor receptor 1, Fms-like tyrosine kinase 2, N-sam, Proto-oncogene c-Fgr, CD331)

035639

200 ul

polyclonal

domestic rabbit

nonconjugated

FGFR1, NT (FGFR1, BFGFR, CEK, FGFBR, FLG, FLT2, HBGFR, Fibroblast growth factor receptor 1, Basic fibroblast growth factor receptor 1, Fms-like tyrosine kinase 2, N-sam, Proto-oncogene c-Fgr, CD331)

rabbit

Pab

Antibodies

200 ul

IgG

FGFR1 is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds both acidic and basic fibroblast growth factors and is involved in limb induction. Mutations in this gene can lead to Pfeiffer syndrome and Jackson-Weiss syndrome. The genomic organization of the gene is very similar to family members 2-4, encompassing 19 exons that are subject to complex alternative splicing, which allows for structural, tissue expression and ligand affinity variations among the isoforms. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:50-1:100 Immunofluorescence: 1:50-1:100 Flow Cytometry: 1:10-1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mo

FGFR1, BFGFR, CEK, FGFBR, FLG, FLT2, HBGFR, Fibroblast growth factor receptor 1, Basic fibroblast growth factor receptor 1, Fms-like tyrosine kinase 2, N-sam, Proto-oncogene c-Fgr, CD331

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 19-48 in the N-terminal region of human FGFR1.

Recognizes human FGFR1. Species Crossreactivity: mouse

Purified by ammonium sulfate precipitation.

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

As Reported

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As Reported Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Purity: Purified by ammonium sulfate precipitation. Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 19-48 in the N-terminal region of human FGFR1. Specificity: Recognizes human FGFR1. Species Crossreactivity: mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E FC IF IHC WB

-20°C

1. Jiao, J., et al., Arch. Biochem. Biophys. 410(2):187-200 (2003). 2. Fu, L., et al., J. Comp. Neurol. 462(2):265-273 (2003). 3. Lundin, L., et al., Exp. Cell Res. 287(1):190-198 (2003). 4. Kiselyov, V.V., et al., Structure (Camb.) 11(6):691-701 (2003). 5. Baumann, H., et al., J. Biol. Chem. 278(18):16198-16208 (2003).

https://usbio-images.r.worldssl.net/prodimages/22/035639_1.jpg

Colocalization of A1B3 and FGFR1 using IF. Confluent ECs (A) or HFFs (B) were treated with or without 100ng/ml FGF-2 in the presence or absence of fibrinogen (10/ml). After 1 hour, cells were washed and fixed with 3.7% formaldehyde and stained using 035639 (10/ml) and 7E3 antibody. FGFR is visualized as red fluorescence (i, iv, vii), A1B3 is visualized as green fluorescence (ii, v, viii), and colocalization of FGF-2 and fibrinogen receptors is shown as yellow fluorescence (iii, vi, ix). Insets represent the background staining for red (i) and green (ii) fluorescence. Bars represent 25.