EZH2 (EZH2, KMT6, Histone-lysine N-methyltransferase EZH2, ENX-1, Enhancer of zeste homolog 2, Lysine N-methyltransferase 6)

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

EZH2 (EZH2, KMT6, Histone-lysine N-methyltransferase EZH2, ENX-1, Enhancer of zeste homolog 2, Lysine N-methyltransferase 6)

catalog :

035287

quantity :

200 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

035287

Product wo Prefix :

EZH2 (EZH2, KMT6, Histone-lysine N-methyltransferase EZH2, ENX-1, Enhancer of zeste homolog 2, Lysine N-methyltransferase 6)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

200 ul

Isotype :

IgG

Desc1 :

EZH2 is a protein that transfers sulfate to the C-4 hydroxyl of beta-1,4-linked GalNAc flanked by GlcUA residues in chondroitin. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:10-1:50 Flow Cytometry: 1:25 Immunofluorescence: 1:25 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mo

Alternate Name :

EZH2, KMT6, Histone-lysine N-methyltransferase EZH2, ENX-1, Enhancer of zeste homolog 2, Lysine N-methyltransferase 6

Immunogen :

Recombinant protein corresponding to a fragment of residues within amino acids1-296 in the N-terminal of human EZH2 (Q15910).

Specificity :

Recognizes human EZH2. Species Crossreactivity: mouse

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Concentration :

As Reported

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As Reported Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Recombinant protein corresponding to a fragment of residues within amino acids1-296 in the N-terminal of human EZH2 (Q15910). Specificity: Recognizes human EZH2. Species Crossreactivity: mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E FC IF IHC WB

Storage Temperature :

-20°C

Reference :

1. Kang,H.G., et.al., J. Biol. Chem. 277 (38), 34766-34772 (2002) 2. Hiraoka,N., et.al., J. Biol. Chem. 275 (26), 20188-20196 (2000)

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/19/035287_1.jpg

Picture4 Text :

Confocal immunofluorescent analysis of 293 cells using 035287 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Picture 5 File Name :

https://usbio-images.r.worldssl.net/prodimages/19/035287_5.jpg

Picture5 Text :

Flow Cytometric analysis of Jurkat cells using 035287 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.