DDIT3, CT (A135) (DDIT3, CHOP, CHOP10, GADD153, DNA damage-inducible transcript 3 protein, C/EBP-homologous protein, C/EBP-homologous protein 10, Growth arrest and DNA damage-inducible protein GADD153)

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product summary

company name :

US Biological

product type :

antibody

product name :

DDIT3, CT (A135) (DDIT3, CHOP, CHOP10, GADD153, DNA damage-inducible transcript 3 protein, C/EBP-homologous protein, C/EBP-homologous protein 10, Growth arrest and DNA damage-inducible protein GADD153)

catalog :

034534

quantity :

200 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

034534

Product wo Prefix :

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

200 ul

Isotype :

IgG

Desc1 :

This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. Applications: Suitable for use in Western Blot, Flow Cytometry, Immunofluorescence and ELISA. Other applications have not been tested. Recommended Dilution: Western Blot: 1:1000-1:2000 Flow Cytometry: 1:10-1:50 Immunofluorescence: 1:10-1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mo

Alternate Name :

DDIT3, CHOP, CHOP10, GADD153, DNA damage-inducible transcript 3 protein, C/EBP-homologous protein, C/EBP-homologous protein 10, Growth arrest and DNA damage-inducible protein GADD153

Immunogen :

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 120-149 in the C-terminal region of human DDIT3. Species sequence homology: bovine, hamster

Specificity :

Recognizes human DDIT3. Species crossreactivity: mouse

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Concentration :

As reported

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As reported Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 120-149 in the C-terminal region of human DDIT3. Species sequence homology: bovine, hamster Specificity: Recognizes human DDIT3. Species crossreactivity: mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E FC IF WB

Storage Temperature :

-20°C

Reference :

Park, S.H., et al. J. Immunol. 185(9):5522-5530(2010) Goodall, J.C., et al. Proc. Natl. Acad. Sci. U.S.A. 107(41):17698-17703(2010) Zhang, H.M., et al. J. Virol. 84(17):8446-8459(2010) Cazanave, S.C., et al. Am. J. Physiol. Gastrointest. Liver Physiol. 299 (1), G236-G243 (2010) : Wang, Y.L., et al. J. Exp. Clin. Cancer Res. 29, 54 (2010) :

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/35/034534_1.png

Picture4 Text :

Fluorescent image of Hela cells stained with 034534. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with 034534 (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). DDIT3 immunoreactivity is localized to Cytoplasm significantly.

Picture 5 File Name :

https://usbio-images.r.worldssl.net/prodimages/35/034534_5.png

Picture5 Text :

Flow Cytometric analysis of 293 cells using 034534 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.