antibody

DAG1, CT (DAG1, Dystroglycan, Dystrophin-associated glycoprotein 1, Alpha-dystroglycan, Beta-dystroglycan) (APC)

034461-APC

200 ul

polyclonal

domestic rabbit

APC

DAG1, CT (DAG1, Dystroglycan, Dystrophin-associated glycoprotein 1, Alpha-dystroglycan, Beta-dystroglycan) (APC)

rabbit

Pab

APC

Antibodies

200 ul

IgG

Dystroglycan is a laminin binding component of the dystrophin-glycoprotein complex which provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. Dystroglycan 1 is a candidate gene for the site of the mutation in autosomal recessive muscular dystrophies. The dramatic reduction of dystroglycan 1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Alternative splicing results in multiple transcript variants all encoding the same protein. Applications: Suitable for use in Western Blot, Immunohistochemistry and FLISA. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Hu Mo

DAG1, Dystroglycan, Dystrophin-associated glycoprotein 1, Alpha-dystroglycan, Beta-dystroglycan

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 718-747 in the C-terminal region of human DAG1, UniProt Accession #Q14118. Species Sequence Homology: porcine and rabbit

Recognizes human DAG1. Species Crossreactivity: mouse

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

As Reported

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As Reported Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC). Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 718-747 in the C-terminal region of human DAG1, UniProt Accession #Q14118. Species Sequence Homology: porcine and rabbit Specificity: Recognizes human DAG1. Species Crossreactivity: mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

FL IHC WB

4°C Do Not Freeze

1. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). 2. Nilsson, J., et al. Glycobiology 20(9):1160-1169(2010). 3. Lara-Chacon, B., et al. J. Cell. Biochem. 110(3):706-717(2010). 4. Sgambato, A., et al. Pathology 42(3):248-254(2010). 5. Masaki, T., et al. J. Biomed. Biotechnol. 2010, 740403 (2010)

https://usbio-images.r.worldssl.net/prodimages/3/034461-APC_1.jpg