antibody

CD73 (NT5E), CT (NT5E, NT5, NTE, 5'-nucleotidase, Ecto-5'-nucleotidase, CD73)

033537

200 ul

polyclonal

domestic rabbit

nonconjugated

CD73 (NT5E), CT (NT5E, NT5, NTE, 5'-nucleotidase, Ecto-5'-nucleotidase, CD73)

rabbit

Pab

Antibodies

200 ul

IgG

Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70 kD subunits bound externally to the plasma membrane by a glycosyl phosphatidyl inositol linkage. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5E occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. It is not known whether the different enzymes are coded by different genes or result from different posttranslational modifications of a single coding sequence. Applications: Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:10-1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mo

NT5E, NT5, NTE, 5'-nucleotidase, Ecto-5'-nucleotidase, CD73

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 520-550 in the C-terminal region of human CD73, UniProt Accession #P21589.

Recognizes human CD73. Species Crossreactivity: mouse

Purified by ammonium sulfate precipitation.

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

As reported

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As reported Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Purity: Purified by ammonium sulfate precipitation. Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 520-550 in the C-terminal region of human CD73, UniProt Accession #P21589. Specificity: Recognizes human CD73. Species Crossreactivity: mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E IHC WB

-20°C

1. Hashikawa, T., et al., J. Dent. Res. 82(11):888-892 (2003). 2. Rosi, F., et al., Biomed. Pharmacother. 56(2):100-104 (2002). 3. Misumi, Y., et al., Eur. J. Biochem. 191(3):563-569 (1990). 4. Boyle, J.M., et al., Hum. Genet. 81(1):88-92 (1988). 5. Kalsi, K., et al., Mol. Cell. Biochem. 232 (1-2), 113-119 (2002).

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Western Blot analysis of lysates from U266B1, HepG2 cell line, mouse brain tissue lysate (from left to right at 20ug/lane), using 033537 (1:2000/lane). A goat anti-rabbit IgG H&L(HRP) at 1:10,000 dilution was used as the secondary antibody.

https://usbio-images.r.worldssl.net/prodimages/0/033537_5.jpg

Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human Placenta tissue reacted with 033537, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.