antibody

CD71, CT (TFRC, Transferrin receptor protein 1, T9, p90, CD71, Transferrin receptor protein 1, serum form) (APC)

033535-APC

200 ul

polyclonal

domestic rabbit

APC

CD71, CT (TFRC, Transferrin receptor protein 1, T9, p90, CD71, Transferrin receptor protein 1, serum form) (APC)

rabbit

Pab

APC

Antibodies

200 ul

IgG

Cellular uptake of iron occurs via receptor mediated endocytosis of ligand occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). Useful in studies of dividing haematopoietic and tumour cell populations, and metabolic activity. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C terminal binding site. The antigen is present on most dividing cells, including normally cycling in vivo hematopoietic progenitor cells, mitogenically stimulated cells in vitro, some primary tumor cells and most proliferating cells in vitro.The transferrin receptor has been structurally characterized as a sulfide bound dimer of identical glycoprotein subunits of 95 kDa. The transferrin receptor is not present on resting blood lymphocytes. On PBL, the receptor appears after activation. The expression of transferrin receptor is coordinately regulated with cell growth. Present on T and B cell lines. The soluble (or serum) transferrin receptor (sTfR) is a circulating truncated form of the membrane receptor protein; it is an 85 kDa glycoprotein forming in serum a 320 kDa complex with diferric transferrin.The most important clinical use of the sTfR determination is in the differential diagnosis between iron deficiency anaemia and the anaemia of chronic disease. This antibody is an indicator of proliferation activity. It also has prognostic significance when typing tumors, such as leukemias and lymphomas. Applications: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, FLISA Recommended Dilution: FLISA: 1:1,000 Western Blot: 1:50-100 Immunohistochemistry: 1:50-100 Flow Cytometry: 1:10-50 Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Hu

TFRC, Transferrin receptor protein 1, T9, p90, CD71, Transferrin receptor protein 1, serum form

CD71 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 656~685 amino acids from the C-terminal region of human CD71.

Human

Purified by immunoaffinity chromatography

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

As reported

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As reported Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC). Purity: Purified by immunoaffinity chromatography. Immunogen: CD71 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 656~685 amino acids from the C-terminal region of human CD71. Specificity: Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

FC FL IHC WB

4°C Do Not Freeze

Chicz,R.M., et.al., Nature 358 (6389), 764-768 (1992) Hayes,G.R., et.al., Glycobiology 2 (4), 355-359 (1992)

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