CD63, CT (CD63, MLA1, TSPAN30, CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, CD63)

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

CD63, CT (CD63, MLA1, TSPAN30, CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, CD63)

catalog :

033528

quantity :

200 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

033528

Product wo Prefix :

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

200 ul

Isotype :

IgG

Desc1 :

CD63 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. The use of alternate polyadenylation sites has been found for this gene. Applications: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry and ELISA. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:50-1:100 Requires HIER using sodium citrate buffer pH6.0 prior to staining. Flow Cytometry: 1:25 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Alternate Name :

CD63, MLA1, TSPAN30, CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, CD63

Immunogen :

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 163-190 in the C-terminal region of human CD63.

Specificity :

Recognizes human CD63.

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Concentration :

As Reported

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As Reported Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 163-190 in the C-terminal region of human CD63. Specificity: Recognizes human CD63. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E FC IHC WB

Storage Temperature :

-20°C

Reference :

Weng, J., et al. J. Virol. 83(15):7467-7474(2009) Kassahn, D., et al. Cell Death Differ. 16(1):115-124(2009) Logozzi, M., et al. PLoS ONE 4 (4), E5219 (2009)

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/1/033528_1.jpg

Picture4 Text :

Immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma using 033528 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of 033528 for immunohistochemistry.

Picture 5 File Name :

https://usbio-images.r.worldssl.net/prodimages/1/033528_5.png

Picture5 Text :

Flow Cytometric analysis of MDA-MB435 cells using 033528 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.