CD55, NT (CD55, CR, DAF, Complement decay-accelerating factor, CD55)

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

CD55, NT (CD55, CR, DAF, Complement decay-accelerating factor, CD55)

catalog :

033524

quantity :

200 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

033524

Product wo Prefix :

CD55, NT (CD55, CR, DAF, Complement decay-accelerating factor, CD55)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

200 ul

Isotype :

IgG

Desc1 :

This gene encodes a protein involved in the regulation of the complement cascade. The encoded glycoprotein is also known as the decay-accelerating factor (DAF); binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM). Two alternatively spliced transcripts encoding different proteins have been identified. The predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. Additional, alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:10-1:50 Immunofluorescence (IC): 1:10-1:50 Flow Cytometry: 1:10-1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Alternate Name :

CD55, CR, DAF, Complement decay-accelerating factor, CD55

Immunogen :

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 51-79 in the N-terminal region of human CD55, UniProt Accession #P08174.

Specificity :

Recognizes human CD55.

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Concentration :

As Reported

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As Reported Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 51-79 in the N-terminal region of human CD55, UniProt Accession #P08174. Specificity: Recognizes human CD55. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E FC IF IHC WB

Storage Temperature :

-20°C

Reference :

1. Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010). 2. Gustafsson, D.J., et al. Virology 405(2):474-482(2010). 3. Alegretti, A.P., et al. Cell. Immunol. 265(2):127-132(2010). 4. Kim, Y., et al. Ann. Clin. Lab. Sci. 40(3):226-232(2010). 5. Storry, J.R., et al. Transfusion 43(3):340-344(2003)

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/27/033524_1.pdf

Picture4 Text :

Flow Cytometric analysis of Hela cells (right histogram) using 033524 compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.