antibody

CD55, NT (CD55, CR, DAF, Complement decay-accelerating factor, CD55) (APC)

033524-APC

200 ul

polyclonal

domestic rabbit

APC

CD55, NT (CD55, CR, DAF, Complement decay-accelerating factor, CD55) (APC)

rabbit

Pab

APC

Antibodies

200 ul

IgG

This gene encodes a protein involved in the regulation of the complement cascade. The encoded glycoprotein is also known as the decay-accelerating factor (DAF); binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM). Two alternatively spliced transcripts encoding different proteins have been identified. The predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. Additional, alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, FLISA Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Hu

CD55, CR, DAF, Complement decay-accelerating factor, CD55

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 51-79 in the N-terminal region of human CD55, UniProt Accession #P08174.

Recognizes human CD55.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

As Reported

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As Reported Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC). Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 51-79 in the N-terminal region of human CD55, UniProt Accession #P08174. Specificity: Recognizes human CD55. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

FC FL IF IHC WB

4°C Do Not Freeze

1. Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010). 2. Gustafsson, D.J., et al. Virology 405(2):474-482(2010). 3. Alegretti, A.P., et al. Cell. Immunol. 265(2):127-132(2010). 4. Kim, Y., et al. Ann. Clin. Lab. Sci. 40(3):226-232(2010). 5. Storry, J.R., et al. Transfusion 43(3):340-344(2003)

https://usbio-images.r.worldssl.net/prodimages/2/033524-APC_1.pdf

Flow Cytometric analysis of Hela cells (right histogram) using 033524 compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.