antibody

CD33 (Siglec 3), NT (CD33, Siglec 3, Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, gp67, CD33)

033496

200 ul

polyclonal

domestic rabbit

nonconjugated

CD33 (Siglec 3), NT (CD33, Siglec 3, Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, gp67, CD33)

rabbit

Pab

Antibodies

200 ul

IgG

SIGLEC3 is a putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. It preferentially binds to alpha2,6-linked sialic acid; the sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, SIGLEC3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. This protein Induces apoptosis in acute myeloid leukemia (in vitro). It has been shown to interact with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation. SIGLEC3 expresses in monocytic/myeloid lineage cells, and contains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in downmodulation of cellular responses. The phosphorylated ITIM motif binds to the SH2 domain of PTPN6/SHP-1. Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. The gene for SIGLEC3 belongs to the immunoglobulin superfamily. Applications: Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications have not been tested. Recommended Dilution: Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:10-1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

CD33, SIGLEC3, Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, gp67, CD33

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 12-39 in the N-terminal region of human SIGLEC3.

Recognizes human SIGLEC3.

Purified by ammonium sulftate precipitation.

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

As reported

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As reported Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Purity: Purified by ammonium sulftate precipitation. Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 12-39 in the N-terminal region of human SIGLEC3. Specificity: Recognizes human SIGLEC3. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E IHC WB

-20°C

Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002). Yousef, G.M., et al., Gene 286(2):259-270 (2002). Vitale, C., et al., Proc. Natl. Acad. Sci. U.S.A. 98(10):5764-5769 (2001). Taylor, V.C., et al., J. Biol. Chem. 274(17):11505-11512 (1999). Ulyanova, T., et al., Eur. J. Immunol. 29(11):3440-3449 (1999).

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