antibody

CD33 (Siglec 3), NT (CD33, Siglec 3, Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, gp67, CD33) (APC)

033496-APC

200 ul

polyclonal

domestic rabbit

APC

CD33 (Siglec 3), NT (CD33, Siglec 3, Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, gp67, CD33) (APC)

rabbit

Pab

APC

Antibodies

200 ul

IgG

SIGLEC3 is a putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. It preferentially binds to alpha2,6-linked sialic acid; the sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, SIGLEC3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. This protein Induces apoptosis in acute myeloid leukemia (in vitro). It has been shown to interact with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation. SIGLEC3 expresses in monocytic/myeloid lineage cells, and contains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in downmodulation of cellular responses. The phosphorylated ITIM motif binds to the SH2 domain of PTPN6/SHP-1. Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. The gene for SIGLEC3 belongs to the immunoglobulin superfamily. Applications: Suitable for use in Western Blot, Immunohistochemistry and FLISA. Other applications have not been tested. Recommended Dilution: Immunohistochemistry: Formalin-fixed, paraffin-embedded sections Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Hu

CD33, SIGLEC3, Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, gp67, CD33

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 12-39 in the N-terminal region of human SIGLEC3.

Recognizes human SIGLEC3.

Purified by Protein A/G affinity chromatography.

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

As reported

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As reported Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC). Purity: Purified by Protein A/G affinity chromatography. Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 12-39 in the N-terminal region of human SIGLEC3. Specificity: Recognizes human SIGLEC3. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

FL IHC WB

4°C Do Not Freeze

Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002). Yousef, G.M., et al., Gene 286(2):259-270 (2002). Vitale, C., et al., Proc. Natl. Acad. Sci. U.S.A. 98(10):5764-5769 (2001). Taylor, V.C., et al., J. Biol. Chem. 274(17):11505-11512 (1999). Ulyanova, T., et al., Eur. J. Immunol. 29(11):3440-3449 (1999).

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