antibody

CASP3 (Asp175) (CASP3, CPP32, Caspase-3, Apopain, Cysteine Protease CPP32, Protein Yama, SREBP Cleavage Activity 1, Caspase-3 Subunit p17, Caspase-3 Subunit p12)

033215

200 ul

polyclonal

domestic rabbit

nonconjugated

CASP3 (Asp175) (CASP3, CPP32, Caspase-3, Apopain, Cysteine Protease CPP32, Protein Yama, SREBP Cleavage Activity 1, Caspase-3 Subunit p17, Caspase-3 Subunit p12)

rabbit

Pab

Antibodies

200 ul

IgG

Caspase-3 (CASP3) is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of the CASP3 gene results in two transcript variants that encode the same protein. Applications: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry and ELISA. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:50-1:100 Flow Cytometry: 1:10-1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Rt

CASP3, CPP32, Caspase-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, Caspase-3 subunit p17, Caspase-3 subunit p12

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 149-179 of human CASP3.

Recognizes human CASP3. Species Crossreactivity: rat

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

As Reported

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As Reported Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 149-179 of human CASP3. Specificity: Recognizes human CASP3. Species Crossreactivity: rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E FC IHC WB

-20°C

1. Mei, Y., et al., Mol. Cell 37(5): 668-678 (2010). 2. Sohn, E.J., et al., Cancer Res. 70(3): 1154-1163 (2010). 3. Karamitopoulou, E., et al., Pathology 42(1): 37-42 (2010). 4. Wang, W., et al., Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 25(11): 1034-1035 (2009).

https://usbio-images.r.worldssl.net/prodimages/16/033215_1.jpg

Flow Cytometric analysis of CEM cells using 033215 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.