BARD1, NT (BARD1, BRCA1-associated RING domain protein 1) (AP) | US Biological 032422-AP product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

BARD1, NT (BARD1, BRCA1-associated RING domain protein 1) (AP)

catalog :

032422-AP

quantity :

200 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

reactivity :

human

product information

Catalog Number :

032422-AP

Product wo Prefix :

BARD1, NT (BARD1, BRCA1-associated RING domain protein 1) (AP)

Host :

rabbit

Product Type :

Pab

Conjugate :

Category :

Antibodies

Size1 :

200 ul

Isotype :

IgG

Desc1 :

BARD1 is a protein which interacts with the N-terminal region of BRCA1. In addition to its ability to bind BRCA1 in vivo and in vitro, it shares homology with the 2 most conserved regions of BRCA1: the N-terminal RING motif and the C-terminal BRCT domain. The RING motif is a cysteine-rich sequence found in a variety of proteins that regulate cell growth, including the products of tumor suppressor genes and dominant protooncogenes. This protein also contains 3 tandem ankyrin repeats. The BARD1/BRCA1 interaction is disrupted by tumorigenic amino acid substitutions in BRCA1, implying that the formation of a stable complex between these proteins may be an essential aspect of BRCA1 tumor suppression. This protein may be the target of oncogenic mutations in breast or ovarian cancer. Applications: Suitable for use in Western Blot, ELISA Recommended Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Storage and Stability: Store product at 4°C. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Calc Crossreactivity :

Alternate Name :

BARD1, BRCA1-associated RING domain protein 1

Immunogen :

BARD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-135 amino acids from the N-terminal region of human BARD1.

Specificity :

Human

Purity :

Purified by Protein A affinity chromatography.

Form :

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phophatase (AP).

Concentration :

As reported

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As reported Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phophatase (AP). Purity: Purified by Protein A affinity chromatography. Immunogen: BARD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-135 amino acids from the N-terminal region of human BARD1. Specificity: Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

4°C Do Not Freeze

Reference :

Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010) Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) : Dizin, E., et al. Int. J. Biochem. Cell Biol. 42(5):693-700(2010) Irminger-Finger, I. Gynecol. Oncol. 117(2):211-215(2010) De Brakeleer, S., et al. Hum. Mutat. 31 (3), E1175-E1185 (2010) :

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/13/032422-AP_1.jpg