ATG5, CT (ATG5, APG5L, ASP, Autophagy protein 5, APG5-like, Apoptosis-specific protein)

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

ATG5, CT (ATG5, APG5L, ASP, Autophagy protein 5, APG5-like, Apoptosis-specific protein)

catalog :

032231

quantity :

200 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

032231

Product wo Prefix :

ATG5, CT (ATG5, APG5L, ASP, Autophagy protein 5, APG5-like, Apoptosis-specific protein)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

200 ul

Isotype :

IgG

Desc1 :

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG5, required for autophagy, conjugates to ATG12 and associates with an isolation membrane to form a cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Applications: Suitable for use in Western Blot, Immunohistochemistry, ELISA Recommended Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mo

Alternate Name :

ATG5, APG5L, ASP, Autophagy protein 5, APG5-like, Apoptosis-specific protein

Immunogen :

ATG5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216~246 amino acids from the C-term of human APG5L.

Specificity :

Human, mouse

Purity :

Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.

Form :

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Concentration :

As reported

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As reported Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS. Immunogen: ATG5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216~246 amino acids from the C-term of human APG5L. Specificity: Human, mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IHC WB

Storage Temperature :

-20°C

Reference :

Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005) Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005) Greenberg JT. Dev Cell. 8(6):799-801. (2005) Levine B. Cell. 120(2):159-62. (2005) Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004) Hammond E.M., et al. FEBS Lett. 425:391-395(1998) Strausberg R.L., et al. PNAS 99:16899-16903(2002) Grand R.J.A., et al. Exp. Cell Res. 218:439-451(1995) Mizushima N., et al. J. Biol. Chem. 273:33889-33892(1998) Mizushima N., et al. J. Cell Biol. 152:657-668(2001)

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/49/032231_1.jpg

Picture4 Text :

Western blot analysis of APG5L (arrow) using rabbit polyclonal APG5L Antibody (P224) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG5L gene.

Picture5 Text :