antibody

ATG12, NT (ATG12, APG12, APG12L, Ubiquitin-like protein ATG12, Autophagy-related protein 12)

032170

200 ul

polyclonal

domestic rabbit

nonconjugated

ATG12, NT (ATG12, APG12, APG12L, Ubiquitin-like protein ATG12, Autophagy-related protein 12)

rabbit

Pab

Antibodies

200 ul

IgG

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG12L is the human homolog of yeast APG12, a ubiquitin-activating enzyme E1-like protein essential for the conjugation system that mediates membrane fusion in autophagy. Applications: Suitable for use in Western Blot, Immunohistochemistry, ELISA Recommended Dilution: ELISA: 1:1,000 Western Blot: 1:100 (Ref.2) Immunohistochemistry: 1:50-100 Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

ATG12, APG12, APG12L, Ubiquitin-like protein ATG12, Autophagy-related protein 12

ATG12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 44~73 amino acids from the N-term of human APG12L.

Human

Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

As reported

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As reported Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS. Immunogen: ATG12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 44~73 amino acids from the N-term of human APG12L. Specificity: Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E IHC WB

-20°C

Yee, K.S. et al. Cell Death Differ. August; 16(8): 1135?145.(2009) Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005) Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005) Greenberg JT. Dev Cell. 8(6):799-801. (2005) Levine B. Cell. 120(2):159-62. (2005) Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004)

https://usbio-images.r.worldssl.net/prodimages/9/032170_1.jpg

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Autophagy APG12L antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Autophagy APG12L antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.