APG5, cleaved (T193) (APG5 Autophagy 5-like, APG5-like, APG5L, Apoptosis-specific Protein, ASP, ATG5 Autophagy-related 5 Homolog, Autophagy Protein 5, hAPG5, Homolog of S Cerevisiae Autophagy 5) (APC) | US Biological 031965-APC product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

APG5, cleaved (T193) (APG5 Autophagy 5-like, APG5-like, APG5L, Apoptosis-specific Protein, ASP, ATG5 Autophagy-related 5 Homolog, Autophagy Protein 5, hAPG5, Homolog of S Cerevisiae Autophagy 5) (APC)

catalog :

031965-APC

quantity :

200 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

APC

reactivity :

human

product information

Catalog Number :

031965-APC

Product wo Prefix :

Host :

rabbit

Product Type :

Pab

Conjugate :

APC

Category :

Antibodies

Size1 :

200 ul

Isotype :

IgG

Desc1 :

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG5, required for autophagy, conjugates to ATG12 and associates with an isolation membrane to form a cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Applications: Suitable for use in Dot Blot, FLISA Recommended Dilution: FLISA1:1,000 Dot blot 1:100-500 Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Calc Crossreactivity :

Immunogen :

Cleaved-APG5-T193 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding pT439 of human APG5.

Specificity :

Human

Purity :

Purified by peptide immunoaffinity chromatography.

Form :

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

Concentration :

As reported

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As reported Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC). Purity: Purified by peptide immunoaffinity chromatography. Immunogen: Cleaved-APG5-T193 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding pT439 of human APG5. Specificity: Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

DB FL

Storage Temperature :

4°C Do Not Freeze

Reference :

Yung, H.W., et al., FEBS Lett. 531(2):168-172 (2002). Tanida, I., et al., Biochem. Biophys. Res. Commun. 296(5):1164-1170 (2002). Tanida, I., et al., J. Biol. Chem. 277(16):13739-13744 (2002). Chen, Y., et al., Yi Chuan Xue Bao 28(11):1077-1084 (2001). Hammond, E.M., et al., FEBS Lett. 425(3):391-395 (1998).