antibody

APG5 (ATG5, APG5L, ASP, Autophagy protein 5, APG5-like, Apoptosis-specific protein) (AP)

031964-AP

200 ul

polyclonal

domestic rabbit

AP

APG5 (ATG5, APG5L, ASP, Autophagy protein 5, APG5-like, Apoptosis-specific protein) (AP)

rabbit

Pab

AP

Antibodies

200 ul

IgG

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG5, required for autophagy, conjugates to ATG12 and associates with an isolation membrane to form a cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Applications: Suitable for use in Western Blot, Immunohistochemistry, ELISA Recommended Dilution: ELISA1:1,000 Western blotting: 1:100-500 Immunohistochemistry: 1:10-50 Storage and Stability: Store product at 4°C. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Hu

ATG5, APG5L, ASP, Autophagy protein 5, APG5-like, Apoptosis-specific protein

APG5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the full length of human APG5.

Human

Purified by Protein G affinity chromatography.

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phophatase (AP).

As reported

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As reported Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phophatase (AP). Purity: Purified by Protein G affinity chromatography. Immunogen: APG5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the full length of human APG5. Specificity: Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E IHC WB

4°C Do Not Freeze

Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005) Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005) Greenberg JT. Dev Cell. 8(6):799-801. (2005) Levine B. Cell. 120(2):159-62. (2005) Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004) Hammond E.M., et al. FEBS Lett. 425:391-395(1998) Strausberg R.L., et al. PNAS 99:16899-16903(2002) Grand R.J.A., et al. Exp. Cell Res. 218:439-451(1995) Mizushima N., et al. J. Biol. Chem. 273:33889-33892(1998) Mizushima N., et al. J. Cell Biol. 152:657-668(2001)

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