antibody

AHSG, CT (AHSG, FETUA, Alpha-2-HS-glycoprotein, Alpha-2-Z-globulin, Ba-alpha-2-glycoprotein, Fetuin-A, Alpha-2-HS-glycoprotein chain A, Alpha-2-HS-glycoprotein chain B)

031717

200 ul

polyclonal

domestic rabbit

nonconjugated

AHSG, CT (AHSG, FETUA, Alpha-2-HS-glycoprotein, Alpha-2-Z-globulin, Ba-alpha-2-glycoprotein, Fetuin-A, Alpha-2-HS-glycoprotein chain A, Alpha-2-HS-glycoprotein chain B)

rabbit

Pab

Antibodies

200 ul

IgG

Alpha2-HS glycoprotein (AHSG), a glycoprotein present in the serum, is synthesized by hepatocytes. The AHSG molecule consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues. However, its exact significance is still obscure. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry and ELISA. Other applications have not been tested. Recommended Dilution: Western Blot: 1:1000 Immunohistochemistry: 1:25 Flow Cytometry: 1:10-1:50 Immunofluorescence: 1:25 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

AHSG, FETUA, Alpha-2-HS-glycoprotein, Alpha-2-Z-globulin, Ba-alpha-2-glycoprotein, Fetuin-A, Alpha-2-HS-glycoprotein chain A, Alpha-2-HS-glycoprotein chain B

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 247-276 in the C-terminal region of human AHSG. UniProt Accession #NP_001613.2; P02765.

Recognizes human AHSG.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

As reported

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As reported Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 247-276 in the C-terminal region of human AHSG. UniProt Accession #NP_001613.2; P02765. Specificity: Recognizes human AHSG. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E FC IF IHC WB

-20°C

1. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) 2. Verduijn, M., et al. Nephrol. Dial. Transplant. (2010) In press : 3. Wang, Y., et al. Zhonghua Yi Xue Yi Chuan Xue Za Zhi 27(3):310-315(2010) 4. Voigt, M., et al. Histopathology 56(6):775-788(2010) 5. Kusnierz-Cabala, B., et al. Clin. Lab. 56 (5-6), 191-195 (2010) :

https://usbio-images.r.worldssl.net/prodimages/42/031717_1.png

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Pdx1 with 1:25 (1:25), followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody (1:200) (green). Immunofluorescence image showing cytoplasm staining on HepG2 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).

https://usbio-images.r.worldssl.net/prodimages/42/031717_5.png

Flow Cytometric analysis of HepG2 cells using 031717 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.