Actin, alpha1, CT (ACTA1, ACTA, Actin, alpha skeletal muscle, Alpha-actin-1)

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Actin, alpha1, CT (ACTA1, ACTA, Actin, alpha skeletal muscle, Alpha-actin-1)

catalog :

031572

quantity :

200 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

031572

Product wo Prefix :

Actin, alpha1, CT (ACTA1, ACTA, Actin, alpha skeletal muscle, Alpha-actin-1)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

200 ul

Isotype :

IgG

Desc1 :

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry and ELISA. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000 Immunofluorescence: 1:10-1:50 Flow Cytometry: 1:10-1:50 Immunohistochemistry (FFPE): 1:10-1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Alternate Name :

ACTA1, ACTA, Actin, alpha skeletal muscle, Alpha-actin-1

Immunogen :

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 346-375 in the C-terminal region of human Actin, alpha1. Species Sequence Homology: bovine, chicken, mouse, porcine, rat, rabbit and Xenopus

Specificity :

Recognizes human Actin, alpha1.

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Concentration :

As Reported

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As Reported Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 346-375 in the C-terminal region of human Actin, alpha1. Species Sequence Homology: bovine, chicken, mouse, porcine, rat, rabbit and Xenopus Specificity: Recognizes human Actin, alpha1. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E FC IF IHC WB

Storage Temperature :

-20°C

Reference :

1. Kim, E.Y., et al. Am. J. Physiol. Renal Physiol. 299 (3), F594-F604 (2010). 2. Haigh, S.E., et al. Neuromuscul. Disord. 20(6):363-374(2010). 3. Yu, G., et al. J Clin Neurosci 7(6):766-769(2010). 4. Yu, C.H., et al. PLoS ONE 5 (7), E11878 (2010). 5. Licastro, F., et al. Curr. Pharm. Des. 16(7):783-788(2010)

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/34/031572_1.jpg

Picture4 Text :

Confocal immunofluorescent analysis of A549 cells using 031572 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Picture 5 File Name :

https://usbio-images.r.worldssl.net/prodimages/34/031572_5.jpg

Picture5 Text :

Flow Cytometric analysis of CEM cells using 031572 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.