antibody

Neurofilament H (Neurofilament Heavy Polypeptide, NF-H, Neurofilament Triplet H Protein, 200kD Neurofilament Protein, NEFH, NFH)

031395

100 ul

monoclonal

mouse

nonconjugated

AH1

The same clone is also sold as:

• Invitrogen: MA5-47473, MA5-47374
• Novus Biologicals: NBP1-05209

Neurofilament H (Neurofilament Heavy Polypeptide, NF-H, Neurofilament Triplet H Protein, 200kD Neurofilament Protein, NEFH, NFH)

mouse

Mab

Antibodies

100 ul

AH1

IgG1,k

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220kD, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (also known as Lou Gehrig's disease) and Alzheimer's disease. 031395 is one of numerous antibodies which reacts preferentially with the axonal phosphorylated forms of NF-H. Interestingly these phosphorylated forms of NF-H are normally restricted to axons, while less phosphorylated forms are found in dendrites. However in numerous damage and disease states, phosphorylated NF-H can be detected with 031395 in dendritic and perikaryal neurofilaments. The HGNC name for this protein is NEFH. Applications: Suitable for use in Immunofluorescence and Western Blot. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:1000 Western Blot: 1:10,000 using chemiluminescence. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Bo Ch Rt

Preparation of native NF-H purified from bovine spinal cord.

Recognizes phosphorylated NF-H KSP (lysine-serine-proline) type sequences. In some species there is some crossreactivity with the related phosphorylated KSP sequences found in the related neurofilament subunit NF-M. Recognizes NF-H strongly in all mammals tested to date and also in chicken. It recognizes neurofilaments in frozen sections in tissue culture and in formalin fixed sections.

Ascites

Supplied as a liquid.

Not determined

Product Type: Mab Isotype: IgG1,k Clone No: AH1 Host: mouse Source: bovine Concentration: Not determined Form: Supplied as a liquid. Purity: Ascites Immunogen: Preparation of native NF-H purified from bovine spinal cord. Specificity: Recognizes phosphorylated NF-H KSP (lysine-serine-proline) type sequences. In some species there is some crossreactivity with the related phosphorylated KSP sequences found in the related neurofilament subunit NF-M. Recognizes NF-H strongly in all mammals tested to date and also in chicken. It recognizes neurofilaments in frozen sections in tissue culture and in formalin fixed sections. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IF WB

-20°C

1. Harris, J., Ayyub, C. and Shaw G. A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62 (1991).

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