VSIG8, CT (V-set and immunoglobulin domain containing 8) | US Biological 031336 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

VSIG8, CT (V-set and immunoglobulin domain containing 8)

catalog :

031336

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

604CT14.1.2

reactivity :

human

product information

Catalog Number :

031336

Product wo Prefix :

VSIG8, CT (V-set and immunoglobulin domain containing 8)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

604CT14.1.2

Isotype :

IgM

Desc1 :

VSIG8 protein expression was observed in the hair follicle at specific stages of the hair cycle, the oral mucosa, and the nail matrix. Expression in the hair follicle and shaft was found in the cuticle and cortex layers of the hair, where the labeling appeared intracellular and at the cell periphery. IHC analysis revealed that VSIG8 was located in the projections of the cortex between medullary cells, a poorly understood structure that is deficient in mice homozygous for the hair interior defect due to a mutation in Soat1 (Wu et al., 2010). To determine whether VSIG8 was expressed at all stages of the hair cycle or only in specific stages and anatomical sites, synchronized (wax-stripped) hair follicles (Sundberg and Silva, in press) were tested. Reactivity was restricted as we initially found in the hair follicle and hair shaft, and positive signals were limited to the late anagen and early catagen stages of the hair cycle. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1:100-1:800 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Immunogen :

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 331-360 in the C-terminal region of human CSIG8, UniProt Accession #Q5VU13.

Specificity :

Recognizes mouse VSIG8.

Purity :

Ascites

Form :

Supplied as a liquid, crude ascites with 0.09% sodium azide.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgM Clone No: 604CT14.1.2 Host: mouse Source: mouse Concentration: Not determined Form: Supplied as a liquid, crude ascites with 0.09% sodium azide. Purity: Ascites Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 331-360 in the C-terminal region of human CSIG8, UniProt Accession #Q5VU13. Specificity: Recognizes mouse VSIG8. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

-20°C

Reference :

General References: 1. Strausberg, R.L., et al. Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002) 2. Journal of Investigative Dermatology (2011) 131, 1936–1938; doi:10.1038/jid.2011.133; published online 26 May 2011, “Localization of Hair Shaft Protein VSIG8 in the Hair Follicle, Nail Unit, and Oral Cavity”, Robert H Rice, Marjorie A Phillips and John P Sundberg

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/30/031336_1.png