antibody

TGFB2 (TGFB2, Transforming growth factor beta-2, BSC-1 cell growth inhibitor, Cetermin, Glioblastoma-derived T-cell suppressor factor, Polyergin)

031281

200 ul

monoclonal

mouse

nonconjugated

220ct16.4.3.1

The same clone is also sold as:

• Invitrogen: MA5-37505
• Abnova: MAB12310

TGFB2 (TGFB2, Transforming growth factor beta-2, BSC-1 cell growth inhibitor, Cetermin, Glioblastoma-derived T-cell suppressor factor, Polyergin)

mouse

Mab

Antibodies

200 ul

220ct16.4.3.1

IgG1,k

This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types by transducing their signal through combinations of transmembrane type I and type II receptors (TGFBR1 and TGFBR2) and their downstream effectors, the SMAD proteins. Disruption of the TGFB/SMAD pathway has been implicated in a variety of human cancers. The encoded protein is secreted and has suppressive effects of interleukin-2 dependent T-cell growth. Translocation t(1;7)(q41;p21) between this gene and HDAC9 is associated with Peters' anomaly, a congenital defect of the anterior chamber of the eye. The knockout mice lacking this gene show perinatal mortality and a wide range of developmental, including cardiac, defects. Alternatively spliced transcript variants encoding different isoforms have been identified. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA. Other applications have not been tested. Recommended Dilution: Western Blot: 1:100-1:1000 Immunohistochemistry (FFPE): 1:10-1:50 Immunofluorescence: 1:10-1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

TGFB2, Transforming growth factor beta-2, BSC-1 cell growth inhibitor, Cetermin, Glioblastoma-derived T-cell suppressor factor, Polyergin

Recombinant protein corresponding to human TGFB2.

Recognizes human TGFB2.

Purified by Protein G affinity chromatography.

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

As reported

Product Type: Mab Isotype: IgG1,k Clone No: 220ct16.4.3.1 Host: mouse Source: human Concentration: As reported Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Purity: Purified by Protein G affinity chromatography. Immunogen: Recombinant protein corresponding to human TGFB2. Specificity: Recognizes human TGFB2. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E IF IHC WB

-20°C

1. Nalpas, B., et al. Gut 59(8):1120-1126(2010) 2. Bailey, S.D., et al. Diabetes Care (2010) In press 3. Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) 4. Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010) 5. Sambo, M.R., et al. PLoS ONE 5 (6), E11141 (2010)

https://usbio-images.r.worldssl.net/prodimages/21/031281_1.png

Immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma using 031281 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of 031281 for immunohistochemistry.

https://usbio-images.r.worldssl.net/prodimages/21/031281_5.jpg

Confocal immunofluorescent analysis of A549 cells using 031281 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).