antibody

Lin28 (Lin-28 Homolog A, Zinc Finger CCHC Domain-containing Protein 1, CSDD1, LIN28A, ZCCHC1) (AP)

031089-AP

200 ul

monoclonal

mouse

AP

55CT58.12.1

The same clone is also sold as:

• OriGene: AM11048PU-N
• Abnova: MAB12278

Lin28 (Lin-28 Homolog A, Zinc Finger CCHC Domain-containing Protein 1, CSDD1, LIN28A, ZCCHC1) (AP)

mouse

Mab

AP

Antibodies

200 ul

55CT58.12.1

IgG1,k

Lin28 is a developmentally regulated RNA binding protein (RBP). It is down regulation is mediated in part by Lin4 and miR-125b, the mammalian micro-RNA homolog of Lin4. It is known to be negatively regulated by retinoic acid and act as a translational enhancer by driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis by increasing the number of initiation events per mRNA molecule. Lin28 is necessary and sufficient for selectively blocking the cleavage processing of pri-let-7 miRNAs in embryonic cells (Viswanathan, 2008). Lin28 is a negative regulator of miRNA biogenesis and may play a role in blocking miRNA-mediated differentiation in stem cells and in certain cancers (Viswanathan, 2008). If this degradation does not occur, then normal development of various somatic cells fails. The mechanism by which miR-125b represses Lin28 in mammalian cells appears to involve reductions not only in translational efficiency, but also in mRNA abundance (Wu, 2005). Lin28 has shown to be essential for skeletal muscle differentiation through the translational upregulation of IGF2 expression. It is known to be expressed in testis, placenta, and embryonic stem (ES) cells. Applications: Suitable for use in Western Blot, Immunohistochemistry, ELISA. Recommended Dilution: ELISA : 1:10000 Western Blot: 1:200~2000 Immunohistochemistry: 1:50~200 Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C with the addition of glycerol. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Note: Applications are based on unconjugated antibody.

Hu

LIN28A recombinant protein.

Human

Purified by Protein G affinity chromatography.

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phophatase (AP).

As reported

Product Type: Mab Isotype: IgG1,k Clone No: 55CT58.12.1 Host: mouse Source: human Concentration: As reported Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phophatase (AP). Purity: Purified by Protein G affinity chromatography. Immunogen: LIN28A recombinant protein. Specificity: Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E IHC WB

4°C Do Not Freeze

Pluripotency factors Lin28 and Oct4 identify a sub-population of stem cell-like cells in ovarian cancer. Peng S, et al. Oncogene, 2010 Apr 8. PMID 20101213. Lin28-mediated post-transcriptional regulation of Oct4 expression in human embryonic stem cells. Qiu C, et al. Nucleic Acids Res, 2010 Mar. PMID 19966271. An epigenetic switch involving NF-kappaB, Lin28, Let-7 MicroRNA, and IL6 links inflammation to cell transformation. Iliopoulos D, et al. Cell, 2009 Nov 13. PMID 19878981. TUT4 in concert with Lin28 suppresses microRNA biogenesis through pre-microRNA uridylation. Heo I, et al. Cell, 2009 Aug 21. PMID 19703396. A role for Lin28 in primordial germ-cell development and germ-cell malignancy. West JA, et al. Nature, 2009 Aug 13. PMID 19578360.

https://usbio-images.r.worldssl.net/prodimages/16/031089-AP_1.jpg